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Updated: Mar 23, 2026

Genome-Wide Analysis of DNA Methylation in Gastrointestinal Cancer
Published on: September 18, 2020
Altered expression of tumor suppressors p16 and Rb in gastric carcinogenesis
1Qi Zhou, Jian-Xiang Zou, Yu-Long Chen, Hui-Zhen Yu, Li-Dong Wang, Yong-Xin Li, Hua-Qin Guo, Shan-Shan Gao, Song-Lian Qiu, Laboratory for Cancer Research, Medical Experimental Center, Zhengzhou 450052, Henan Province, China.
Aim:
To determine whether expression of the tumor suppressors p16 and Rb is altered in gastric carcinoma.
Methods:
Mucosal biopsies were endoscopically obtained from patients with superficial gastritis (n = 12), atrophic gastritis (n = 15), atypical hyperplasia (n = 20) and gastric cancer (n = 40). Upon obtainment, all samples were immediately fixed with 10% buffered formalin, embedded in paraffin, and sectioned serially. Protein expression of p16 and Rb was detected by immunohistochemistry (ABC method).
Results:
The gastric epithelium samples showed various degrees of nuclear immunostaining for p16 and Rb according to the different stage of lesion. Progressive pathology of the lesions was associated with a decreasing trend in positive immunostaining for p16 protein (83.3% > 73.3% > 30.0% > 27.5%) but an increasing trend for Rb protein (25.0% > 46.7% > 60.0% > 67.5%). A negative correlation was found between these two parameters and gastric cancer. Correlation analysis of the 40 cases of gastric cancer identified a negative correlation for 20 of the cases. When positive (n = 9) and negative tissues (n = 11) were compared, a statistically significant difference was found (50.0%, 22.5%, 27.5%) (P < 0.05).
Conclusion:
Abnormal expression of p16 and Rb may play an important role in gastric carcinogenesis.
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