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DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
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Combinatorial Dansyl Library and its Applications to pH-Responsive Probes.

Seong Cheol Hong, Dhiraj P Murale, Jun-Seok Lee¹

¹Molecular Recognition Research Center, Korea Institute of Science and Technology (KIST), P.O. Box: 136-791, Seoul, Republic of Korea. junseoklee@kist.re.kr.

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|April 9, 2016

Summary

Researchers developed a novel library of 48 fluorescent probes based on dansyl chemistry. These probes exhibit distinct pH responses and cell permeability, showing potential for biosensor and bio-imaging applications.

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Area of Science:

Organic chemistry
Analytical chemistry
Biochemistry

Background:

Fluorescent probes are crucial tools in biological and chemical research.
Developing novel fluorescent libraries with tunable properties is essential for advancing sensing technologies.
Dansyl-based compounds offer unique photophysical characteristics suitable for probe development.

Purpose of the Study:

To synthesize and characterize the first 48-membered, dansyl-based, combinatorial fluorescent library.
To evaluate the optical properties, chemical yields, and pH responsiveness of the synthesized probes.
To assess the cell permeability of selected probes for potential biological applications.

Main Methods:

Combinatorial synthesis of a dansyl-based fluorescent library.
Analysis of electronic and structural properties to predict optical behavior.
Spectroscopic measurements to determine optical properties and pH responses.
Cell permeability assays using SNU-2292 cells.

Main Results:

Successful creation of a 48-member dansyl-based fluorescent library with an average chemical yield of 49%.
Probes DS-2 and DS-45 exhibited blue-shifts, while DS-7 and DS-40 showed red-shifts in wavelength with increasing pH.
Demonstrated cell permeability of the developed fluorescent probes.

Conclusions:

The novel dansyl-based fluorescent library possesses diverse optical properties and pH sensitivity.
The library holds significant potential for applications in developing advanced biosensors, bio-imaging agents, and pH indicators.