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A Fluorescence Turn-On Probe for Thiols with a Tunable Dynamic Range.

Qian Li1, Rui Guo1, Weiying Lin2,3

  • 1State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha, Hunan, 410082, People's Republic of China.

Journal of Fluorescence
|April 9, 2016
PubMed
Summary

A new Coumarin-Rhodamine based probe detects thiols using a FRET system. The probe, Cou-Rho-SA-Cu (II), shows fluorescence changes in response to glutathione (GSH) levels.

Keywords:
Cu2+ complexDynamic rangeFRETFluorescent probeThiols

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Area of Science:

  • Analytical Chemistry
  • Biochemistry
  • Materials Science

Background:

  • Thiols, such as glutathione (GSH), are crucial biomolecules involved in various cellular processes.
  • Developing sensitive and selective probes for thiol detection is essential for biological and medical research.
  • Existing methods for thiol detection may lack sensitivity or specificity.

Purpose of the Study:

  • To design and synthesize a novel fluorescent probe for the detection of thiols.
  • To investigate the mechanism of action of the probe based on a coumarin-rhodamine Förster Resonance Energy Transfer (FRET) system.
  • To demonstrate the probe's ability to detect varying levels of glutathione.

Main Methods:

  • Synthesis of the Cou-Rho-SA probe.
  • Formation of the Cou-Rho-SA-Cu (II) complex.
  • Fluorescence spectroscopy to monitor probe response.
  • Investigation of FRET efficiency and the role of Cu(2+).

Main Results:

  • The Cou-Rho-SA probe exhibited strong blue fluorescence in its ring-closed form without FRET.
  • The Cou-Rho-SA-Cu (II) complex showed quenched fluorescence due to FRET and Cu(2+) paramagnetic effects.
  • Addition of GSH restored the rhodamine moiety, inhibited FRET, and re-induced blue fluorescence.
  • The probe demonstrated tunable sensitivity to different GSH concentrations by adjusting Cu(2+) levels.

Conclusions:

  • A novel Cou-Rho-SA-Cu (II) probe was successfully developed for thiol detection.
  • The probe utilizes a coumarin-rhodamine FRET system and Cu(2+) interaction for sensitive detection of GSH.
  • The probe's fluorescence response allows for quantitative detection of varying thiol concentrations.