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Bioreactors are engineered vessels designed to cultivate microorganisms under controlled conditions for industrial bioprocessing. They maintain sterility and allow precise regulation of pH, temperature, oxygen, and nutrient levels to optimize microbial growth and metabolite production. Bioreactors range from small laboratory units of 1 liter to industrial systems holding up to 500,000 liters, though only about 75% of their volume is actively used for fermentation. The remaining headspace...
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Upstream processing represents a critical phase in biomanufacturing, wherein biological systems such as microorganisms, mammalian cells, or insect cells are cultivated to produce therapeutic proteins, vaccines, enzymes, or other biologically derived products. This phase encompasses all steps from the selection and genetic manipulation of the production organism to the cultivation of cells in bioreactors under tightly controlled environmental conditions.Host Selection and Genetic OptimizationThe...
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Enhancing Photon Utilization Efficiency for Astaxanthin Production from Haematococcus lacustris Using a Split-Column

Z-Hun Kim1, Hanwool Park1, Ho-Sang Lee1

  • 1National Marine Bioenergy R&D Center & Department of Biological Engineering, Inha University, Incheon 22212, Republic of Korea.

Journal of Microbiology and Biotechnology
|April 9, 2016
PubMed
Summary

A novel split-column photobioreactor (SC-PBR) significantly boosts astaxanthin production from Haematococcus lacustris. This innovative design improves photon utilization efficiency by up to 366% compared to standard systems.

Keywords:
Bubble-column photobioreactorHaematococcusastaxanthinliquid circulationphoton efficiency

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Area of Science:

  • Biotechnology
  • Algal Biotechnology
  • Photosynthesis Research

Background:

  • Haematococcus lacustris is a microalga known for astaxanthin production.
  • Efficient light utilization is crucial for optimizing microalgal cultivation.
  • Standard photobioreactors face challenges in maximizing photon efficiency.

Purpose of the Study:

  • To develop and evaluate a split-column photobioreactor (SC-PBR) for enhanced astaxanthin production.
  • To improve photon utilization efficiency in Haematococcus lacustris cultivation.
  • To compare the performance of SC-PBR with a standard bubble-column photobioreactor (BC-PBR).

Main Methods:

  • Designed a split-column photobioreactor with two columns of different sizes, illuminating only the smaller column.
  • Cultivated Haematococcus lacustris in both SC-PBR and BC-PBR systems.
  • Measured and compared astaxanthin productivity and photon utilization efficiencies between the two reactor types.

Main Results:

  • The SC-PBR demonstrated a 28% improvement in astaxanthin productivity compared to the BC-PBR.
  • Photon utilization efficiencies in the SC-PBR were 28-366% higher than in the BC-PBR.
  • The targeted illumination strategy in SC-PBR effectively enhanced astaxanthin yield.

Conclusions:

  • The split-column photobioreactor design is effective for increasing astaxanthin production.
  • Optimized light regimes, as achieved by SC-PBR, significantly enhance algal productivity.
  • SC-PBR offers a promising approach for more efficient astaxanthin biosynthesis in microalgae.