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Inhibition of rat heart and liver microsomal lipid peroxidation by nifedipine
1Department of Basic Pharmaceutical Sciences, College of Pharmacy, University of South Carolina, Columbia 29208.
Abstract:
Lipid peroxidation in rat heart and liver microsomes was induced by an NADPH-generating system or by ascorbate in the presence of an ADP-iron complex. Microsomal lipid peroxidation, as measured by malonaldehyde formation, was inhibited by nifedipine over a wide range of concentrations (47 microM to 6 mM). Nifedipine also decreased the oxygen consumption of cardiac and hepatic microsomes in a concentration-dependent manner. These results indicate that nifedipine may perturb microsomal electron transport systems. Nifedipine may have the potential to alter the sensitivity of cardiac and hepatic membranes to peroxidative damage.
Insights
Nifedipine inhibits lipid peroxidation in rat heart and liver microsomes, suggesting it may alter cellular membrane sensitivity to oxidative damage by perturbing electron transport systems.
Area of Science:
- Biochemistry
- Pharmacology
- Cellular Biology
Background:
- Lipid peroxidation is a key process in oxidative stress.
- Microsomes are critical cellular components involved in metabolic processes.
- Understanding drug interactions with cellular mechanisms is vital.
Purpose of the Study:
- To investigate the effect of nifedipine on lipid peroxidation in rat heart and liver microsomes.
- To explore the potential mechanisms by which nifedipine influences microsomal activity.
Main Methods:
- Lipid peroxidation was induced using an NADPH-generating system or ascorbate with an ADP-iron complex.
- Malonaldehyde formation was measured to quantify lipid peroxidation.
- Oxygen consumption in microsomes was monitored in the presence of nifedipine.
Main Results:
- Nifedipine significantly inhibited microsomal lipid peroxidation across a broad concentration range.
- Nifedipine dose-dependently decreased oxygen consumption in both cardiac and hepatic microsomes.
- These findings suggest nifedipine interferes with microsomal electron transport.
Conclusions:
- Nifedipine demonstrates inhibitory effects on lipid peroxidation in rat heart and liver microsomes.
- The drug appears to perturb microsomal electron transport systems.
- Nifedipine may modulate the susceptibility of cardiac and hepatic membranes to peroxidative damage.