Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Electron Transport Chain: Complex I and II01:46

Electron Transport Chain: Complex I and II

19.5K
The mitochondrial electron transport chain (ETC) is the main energy generation system in the eukaryotic cells. However, mitochondria also produce cytotoxic reactive oxygen species (ROS) due to the large electron flow during oxidative phosphorylation. While Complex I is one of the primary sources of superoxide radicals, ROS production by Complex II is uncommon and may only be observed in cancer cells with mutated complexes.
ROS generation is regulated and maintained at moderate levels necessary...
19.5K
Mitochondria01:37

Mitochondria

21.2K
Mitochondria are eukaryotic cellular organelles that are known to produce energy through a process called oxidative phosphorylation. Besides their primary function, mitochondria are involved in various cellular processes, including cell growth, differentiation, signaling, metabolism, and senescence. Age-related changes cause a decline in mitochondrial quality and integrity due to increased mitochondrial mutations and oxidative damage. Thus, aging can severely impact mitochondrial functions,...
21.2K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Dysregulation of the urea cycle - potential targets for treatment and diagnosis in prostate cancer.

Expert reviews in molecular medicine·2026
Same author

"Alternative" or integrated role of mitochondrial glycerol-3-phosphate dehydrogenase.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same author

Multimodal imaging and molecular analysis of a 350-year-old purple heron mummy.

Scientific reports·2026
Same author

Austrian Real-world Experience With Standard of Care Brexucabtagene Autoleucel in Patients With Relapsed or Refractory Mantle-Cell Lymphoma.

Transplantation and cellular therapy·2026
Same author

Pitfalls of onco-metabolomics: impact of sample integrity on metabolomic investigations in more than 4500 human serum samples from ten different cohorts.

Frontiers in molecular biosciences·2026
Same author

Ferric carboxymaltose increases fracture risk in patients and reduces bone formation in mice with iron deficiency anemia.

Blood·2026

Related Experiment Video

Updated: Mar 22, 2026

Analytical Determination of Mitochondrial Function of Excised Solid Tumor Homogenates
11:32

Analytical Determination of Mitochondrial Function of Excised Solid Tumor Homogenates

Published on: August 6, 2021

3.1K

Oxidative phosphorylation and mitochondrial function differ between human prostate tissue and cultured cells.

Bernd Schöpf1, Georg Schäfer2,3, Anja Weber2

  • 1Division of Genetic Epidemiology, Department of Medical Genetics, Molecular and Clinical Pharmacology, Medical University of Innsbruck, Austria.

The FEBS Journal
|April 10, 2016
PubMed
Summary

This study developed a method to analyze mitochondrial respiration in small prostate tissue samples. Cell lines partially mimic tissue bioenergetics, highlighting differences in metabolic substrate utilization.

Keywords:
electron transfer systemhigh-resolution respirometrymitochondriaoxidative phosphorylationprostate metabolism

More Related Videos

Author Spotlight: Oxygen-Independent Assays to Measure Mitochondrial Function in Mammals
05:59

Author Spotlight: Oxygen-Independent Assays to Measure Mitochondrial Function in Mammals

Published on: May 19, 2023

3.6K
Author Spotlight: Advancing Male Infertility Research by Unraveling Sperm Metabolism and Mitochondrial Function
08:32

Author Spotlight: Advancing Male Infertility Research by Unraveling Sperm Metabolism and Mitochondrial Function

Published on: June 23, 2023

3.1K

Related Experiment Videos

Last Updated: Mar 22, 2026

Analytical Determination of Mitochondrial Function of Excised Solid Tumor Homogenates
11:32

Analytical Determination of Mitochondrial Function of Excised Solid Tumor Homogenates

Published on: August 6, 2021

3.1K
Author Spotlight: Oxygen-Independent Assays to Measure Mitochondrial Function in Mammals
05:59

Author Spotlight: Oxygen-Independent Assays to Measure Mitochondrial Function in Mammals

Published on: May 19, 2023

3.6K
Author Spotlight: Advancing Male Infertility Research by Unraveling Sperm Metabolism and Mitochondrial Function
08:32

Author Spotlight: Advancing Male Infertility Research by Unraveling Sperm Metabolism and Mitochondrial Function

Published on: June 23, 2023

3.1K

Area of Science:

  • Mitochondrial physiology
  • Cancer metabolism
  • Prostate tissue analysis

Background:

  • Mitochondrial dysfunction is implicated in diseases like cancer.
  • Cell lines are common models, but their metabolic representation of primary tissue is unclear.
  • Understanding tissue-specific mitochondrial function is crucial for disease research.

Purpose of the Study:

  • To develop and validate a method for assessing oxidative phosphorylation in small prostate tissue biopsies.
  • To compare the mitochondrial respiration and metabolic properties of prostate cell lines with primary tissue.
  • To investigate substrate-specific respiratory differences between prostate tissue and cell lines.

Main Methods:

  • High-resolution respirometry was used to analyze oxidative phosphorylation (OXPHOS) in prostate tissue and cell lines.
  • A novel method for preparing small (2 mg) mechanically permeabilized prostate tissue biopsies was established.
  • Comparative analysis of respiratory activity using various substrates (succinate, glutamate, pyruvate) and zinc levels.

Main Results:

  • Mechanical permeabilization is a reliable method for OXPHOS analysis in small prostate tissue samples.
  • Prostate cell lines (RWPE1, NAF) partially represent tissue bioenergetics but show significant differences.
  • Tissue exhibited higher oxidative capacity with succinate/glutamate, while cell lines showed higher activity with pyruvate.
  • Elevated zinc levels in tissue supported the role of aconitase in prostate-specific metabolism.

Conclusions:

  • Mechanical permeabilization enables reliable respirometry on small prostate biopsy samples.
  • Cell lines offer limited insight into the specific bioenergetic profile of prostate tissue.
  • Combining tissue and cell line analysis provides comprehensive tools for studying mitochondrial function and disease-specific metabolism.