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High-Pressure NMR Experiments for Detecting Protein Low-Lying Conformational States
Published on: June 29, 2021
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High pressure effects on the structural functionality of condensed globular-protein matrices
Sobhan Savadkoohi1, Stefan Kasapis1
1School of Science, RMIT University, Bundoora West Campus, Plenty Road, Vic 3083, Australia.
International Journal of Biological Macromolecules
|April 10, 2016
Summary
High hydrostatic pressure (HPP) processing of condensed globular proteins creates stable biomaterials. This technology effectively reduces pathogens and enzymes, offering new possibilities for functional foods and protein ingredients.
Area of Science:
- Food Science and Technology
- Biomaterials Engineering
- Protein Chemistry
Background:
- Consumer demand drives innovation in food quality control.
- High pressure processing (HPP) shows potential for advanced biomaterials from proteins.
- Previous research focused on low-solid protein systems.
Purpose of the Study:
- To explore the application of HPP to condensed globular protein systems.
- To generate industry-relevant biomaterials with enhanced techno- and biofunctionality.
- To review fundamental concepts and structural functionality of condensed protein matrices.
Main Methods:
- Application of high hydrostatic pressure to condensed globular protein systems.
- Microbiological challenge tests on high-solid protein samples.
- Analysis of structural integrity and native conformation preservation.
Main Results:
- HPP generates coherent structures in condensed globular proteins, preserving native conformation.
- HPP effectively reduces foodborne pathogens (Gram-negative and Gram-positive) and proteolytic enzymes.
- Condensed HPP-treated protein pastes maintain structure and bioactivity, unlike spray-dried powders.
Conclusions:
- HPP offers a novel method for creating advanced biomaterials from condensed proteins.
- This technology has significant industrial implications for functional foods and protein ingredients.
- HPP provides a promising alternative to traditional methods like spray drying for protein stabilization.
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