Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Epigenetic Regulation01:37

Epigenetic Regulation

4.1K
Epigenetic changes alter the physical structure of the DNA without changing the genetic sequence and often regulate whether genes are turned on or off. This regulation ensures that each cell produces only proteins necessary for its function. For example, proteins that promote bone growth are not produced in muscle cells. Epigenetic mechanisms play an essential role in healthy development. Conversely, precisely regulated epigenetic mechanisms are disrupted in diseases like cancer.
X-chromosome...
4.1K
Epigenetic Regulation01:46

Epigenetic Regulation

34.2K
Epigenetic mechanisms play an essential role in healthy development. Conversely, precisely regulated epigenetic mechanisms are disrupted in diseases like cancer.
34.2K
Phase II Reactions: Methylation Reactions01:17

Phase II Reactions: Methylation Reactions

863
Methylation is a phase II biotransformation process involving the attachment of a methyl group to a substrate. Enzymes known as methyltransferases orchestrate this reaction.
The mechanism of methylation unfolds in two stages. The first stage sees a methyltransferase enzyme facilitating the transfer of a methyl group from S-adenosylmethionine (SAM) to the substrate, forming S-adenosylhomocysteine (SAH). The second stage involves further metabolism of SAH into homocysteine, which can be recycled...
863
Pharmacogenetics of Drug Targets: β₂-Adrenergic Receptors, Apo E, Thymidylate Synthase01:11

Pharmacogenetics of Drug Targets: β₂-Adrenergic Receptors, Apo E, Thymidylate Synthase

65
Genetic polymorphisms in drug targets have emerged as critical determinants of interindividual variability in drug response and toxicity. Pharmacogenomic investigations increasingly focus on identifying these variations to personalize and optimize therapeutic interventions. A drug target may be a receptor, enzyme, or signaling protein involved in pharmacologic responses or disease-related pathways. While early pharmacogenetic studies focused primarily on drug metabolism, current research...
65
Genomic Imprinting and Inheritance02:30

Genomic Imprinting and Inheritance

38.6K
Diploid organisms inherit genetic material through chromosomes from both parents. Copies of the same gene are known as alleles. In most cases, both alleles are simultaneously expressed and allow various cellular processes to function optimally. If one of the alleles is missing or mutated, the expression of the other allele can compensate; however, this is not true for all genes.
The expression of some genes depends on which parent passed the gene to the offspring, through a phenomenon known as...
38.6K
Pharmacogenetics of Phase II Enzymes: N-acetyltransferase, Thiopurine S-methyltransferase, UDP-glucuronosyltransferase01:27

Pharmacogenetics of Phase II Enzymes: N-acetyltransferase, Thiopurine S-methyltransferase, UDP-glucuronosyltransferase

58
Phase II biotransformation reactions are essential for detoxifying and eliminating xenobiotics, including many pharmaceutical compounds. These reactions typically involve conjugation, the covalent attachment of polar endogenous groups such as glucuronic acid, sulfate, methyl, or acetyl moieties to functional groups introduced during Phase I metabolism. The resulting conjugates are more water-soluble, enabling efficient renal or biliary excretion.The major classes of Phase II enzymes include...
58

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Subtype matters: ovarian endometriosis impairs ovarian reserve and embryo quality-should these patients consider fertility preservation?

Archives of gynecology and obstetrics·2026
Same author

Deep molecular profiling of biliary tract cancer uncovers novel biological mechanisms and therapeutic opportunities.

ESMO open·2026
Same author

Clinical outcomes of drug-coated balloon vs. second-generation drug-eluting stent for coronary in-stent restenosis.

Clinical research in cardiology : official journal of the German Cardiac Society·2025
Same author

In vivo biomarkers of structural and functional brain development and aging in humans.

Neuroscience and biobehavioral reviews·2020
Same author

Association Between Systemic Inflammation, Carotid Arteriosclerosis, and Autonomic Dysfunction.

Translational stroke research·2019
Same author

CORRIGENDUM: Implementing Pharmacogenomics in Europe: Design and Implementation Strategy of the Ubiquitous Pharmacogenomics Consortium.

Clinical pharmacology and therapeutics·2018

Related Experiment Video

Updated: Mar 22, 2026

Continuous Fluorescence-Based Endonuclease-Coupled DNA Methylation Assay to Screen for DNA Methyltransferase Inhibitors
06:07

Continuous Fluorescence-Based Endonuclease-Coupled DNA Methylation Assay to Screen for DNA Methyltransferase Inhibitors

Published on: August 5, 2022

3.2K

DNA Methylation of ADME Genes.

P Fisel1,2, E Schaeffeler1,2, M Schwab1,3,4

  • 1Dr. Margarete Fischer-Bosch Institute of Clinical Pharmacology, Stuttgart, Germany.

Clinical Pharmacology and Therapeutics
|April 11, 2016
PubMed
Summary

Epigenetic regulation, specifically DNA methylation, impacts how individuals process drugs (ADME). Understanding these epigenetic changes is key to predicting drug responses and reducing toxicity for personalized medicine.

More Related Videos

Immunostaining for DNA Modifications: Computational Analysis of Confocal Images
09:42

Immunostaining for DNA Modifications: Computational Analysis of Confocal Images

Published on: September 7, 2017

10.4K
Enhanced Reduced Representation Bisulfite Sequencing for Assessment of DNA Methylation at Base Pair Resolution
13:47

Enhanced Reduced Representation Bisulfite Sequencing for Assessment of DNA Methylation at Base Pair Resolution

Published on: February 24, 2015

26.6K

Related Experiment Videos

Last Updated: Mar 22, 2026

Continuous Fluorescence-Based Endonuclease-Coupled DNA Methylation Assay to Screen for DNA Methyltransferase Inhibitors
06:07

Continuous Fluorescence-Based Endonuclease-Coupled DNA Methylation Assay to Screen for DNA Methyltransferase Inhibitors

Published on: August 5, 2022

3.2K
Immunostaining for DNA Modifications: Computational Analysis of Confocal Images
09:42

Immunostaining for DNA Modifications: Computational Analysis of Confocal Images

Published on: September 7, 2017

10.4K
Enhanced Reduced Representation Bisulfite Sequencing for Assessment of DNA Methylation at Base Pair Resolution
13:47

Enhanced Reduced Representation Bisulfite Sequencing for Assessment of DNA Methylation at Base Pair Resolution

Published on: February 24, 2015

26.6K

Area of Science:

  • Pharmacogenomics
  • Epigenetics
  • Drug Metabolism

Background:

  • Interindividual variability in drug response is a significant challenge in clinical practice.
  • Epigenetic mechanisms, including DNA methylation, histone modifications, and microRNAs, play a crucial role in regulating gene expression.
  • Genes involved in the absorption, distribution, metabolism, and excretion (ADME) of drugs are subject to epigenetic control.

Purpose of the Study:

  • To systematically review the influence of DNA methylation on ADME gene expression.
  • To highlight the consequences of DNA methylation-mediated changes in ADME genes on drug response variability and drug-induced toxicity.
  • To discuss the implications of these findings for the advancement of personalized medicine.

Main Methods:

  • Systematic literature review.
  • Analysis of studies investigating DNA methylation patterns in ADME genes.
  • Integration of findings related to drug response and toxicity.

Main Results:

  • DNA methylation significantly influences the expression of numerous ADME genes.
  • Altered DNA methylation patterns in ADME genes are associated with substantial interindividual differences in drug efficacy and safety.
  • Specific examples of DNA methylation affecting key drug-metabolizing enzymes and transporters are discussed.

Conclusions:

  • DNA methylation is a critical determinant of ADME gene expression and subsequent drug response variability.
  • Targeting epigenetic modifications, particularly DNA methylation, holds promise for personalized therapeutic strategies.
  • Further research into epigenetic regulation of ADME genes is essential for optimizing drug therapy and minimizing adverse drug reactions.