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Updated: Mar 22, 2026

Identification of Rare Bacterial Pathogens by 16S rRNA Gene Sequencing and MALDI-TOF MS
Published on: July 11, 2016
Performance of MALDI-TOF MS platforms for fungal identification
Carole Cassagne1,2, Anne-Cécile Normand3, Coralie L'Ollivier3,4
1Parasitology and Mycology, Assistance Publique-Hôpitaux de Marseille, CHU Timone-Adultes, Marseilles CEDEX 5, France. carole.cassagne@ap-hm.fr.
Abstract:
Matrix-assisted laser desorption/ionisation mass spectrometry (MALDI-TOF MS) is increasingly used by clinical microbiology laboratories to cope with the need for rapid, cost-effective and accurate identification of microorganisms. Several research teams have recently succeed in identifying moulds using MALDI-TOF MS, which was first adapted to bacteria, then to yeast identification. Since 2004, different commercial firms have released several ready-to-use MALDI-TOF MS platforms. This review describes the similarities and differences between the commercially available systems. In two parts, we first describe and compare the preprocessing and identification steps between the platforms and then compare the identification efficacy of yeast, moulds and dermatophytes species.
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