Related Experiment Video

Updated: Mar 22, 2026

In Vitro Cultivation Techniques for Modeling Liver Organogenesis, Building Assembloids, and Designing Synthetic Tissues using Human Cell Lines
08:50

In Vitro Cultivation Techniques for Modeling Liver Organogenesis, Building Assembloids, and Designing Synthetic Tissues using Human Cell Lines

Published on: April 18, 2025

1.1K

Mir-765 promotes cell proliferation by downregulating INPP4B expression in human hepatocellular carcinoma

Bin-Hui Xie1, Xiao He1, Rui-Xi Hua2

  • 1Department of General Surgery, the First Affiliated Hospital of Gannan Medical University, Guangzhou, Guangdong, China.

Cancer Biomarkers : Section a of Disease Markers
|April 11, 2016
PubMed

Insights

MicroRNA-765 (miR-765) promotes hepatocellular carcinoma (HCC) growth by targeting INPP4B. Inhibiting miR-765 may offer a new therapeutic strategy for HCC patients.

Area of Science:

  • Oncology
  • Molecular Biology
  • Genetics

Background:

  • MicroRNA (miRNA) dysregulation is implicated in cancer progression, including sustained cell proliferation.
  • Understanding miRNA mechanisms in carcinogenesis is crucial for improving cancer diagnostics and therapeutics.

Purpose of the Study:

  • To investigate the role of miR-765 in hepatocellular carcinoma (HCC) development.
  • To identify the molecular targets and pathways regulated by miR-765 in HCC.

Main Methods:

  • Quantitative real-time PCR to assess miR-765 expression in HCC cell lines and tissues.
  • Cell proliferation and tumorigenicity assays following miR-765 overexpression or inhibition.
  • Western blotting to analyze protein expression levels of INPP4B, AKT, Cyclin D1, FOXO3a, and p21.

Main Results:

  • miR-765 was significantly upregulated in HCC cell lines and tissues compared to normal controls.
  • Overexpression of miR-765 enhanced HCC cell proliferation and tumorigenicity, while inhibition reversed these effects.
  • miR-765 directly targets INPP4B, leading to decreased INPP4B expression and subsequent modulation of AKT, Cyclin D1, FOXO3a, and p21 signaling pathways.

Conclusions:

  • miR-765 acts as an onco-microRNA in human HCC by suppressing INPP4B expression.
  • miR-765 plays a critical role in HCC cell proliferation through the miR-765/INPP4B axis.
  • miR-765 represents a potential diagnostic biomarker and therapeutic target for HCC.
Keywords:
INPP4Bhuman hepatocellular carcinomamiR-765proliferation

More Related Videos

An In Vitro Protocol for Evaluating MicroRNA Levels, Functions, and Associated Target Genes in Tumor Cells09:45

An In Vitro Protocol for Evaluating MicroRNA Levels, Functions, and Associated Target Genes in Tumor Cells

Published on: May 21, 2019

9.0K
In Vivo Inhibition of MicroRNA to Decrease Tumor Growth in Mice07:02

In Vivo Inhibition of MicroRNA to Decrease Tumor Growth in Mice

Published on: August 23, 2019

7.9K

Related Concept Videos

Abnormal Proliferation02:23

Abnormal Proliferation

Under normal conditions, most adult cells remain in a non-proliferative state unless stimulated by internal or external factors to replace lost cells. Abnormal cell proliferation is a condition in which the cell's growth exceeds and is uncoordinated with normal cells. In such situations, cell division persists in the same excessive manner even after cessation of the stimuli, leading to persistent tumors. The tumor arises from the damaged cells that replicate to pass the damage to the...
5.4K
Inhibition of Cdk Activity02:34

Inhibition of Cdk Activity

The orderly progression of the cell cycle depends on the activation of Cdk protein by binding to its cyclin partner. However, the cell cycle must be restricted when undergoing abnormal changes. Most cancers correlate to the deregulated cell cycle, and since Cdks are a central component of the cell cycle, Cdk inhibitors are extensively studied to develop anticancer agents. For instance, cyclin D associates with several Cdks, such as Cdk 4/6, to form an active complex. The cyclin D-Cdk4/6 complex...
6.2K
MicroRNAs01:22

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...
4.2K