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Published on: March 14, 2019
LOC100287225, novel long intergenic non-coding RNA, misregulates in colorectal cancer
Mina Kazemzadeh1, Reza Safaralizadeh1, Mohammad Ali Hosseinpour Feizi1
1Department of Animal Biology, Faculty of Natural Sciences, University of Tabriz, Tabriz, Iran.
Abstract:
Colorectal cancer (CRC) is one of the most common cancers in the world; therefore, extensive research is needed to find new molecular therapeutic targets and biomarkers. LncRNA (long non-coding RNA), a new class of non-coding RNAs, has a crucial role in the onset and progression of various cancers including colorectal cancer. Research on lncRNA is still at initial stages and underlying molecular mechanisms of the vast majority of lncRNA have remained unclear. LOC100287225 is one of these novel lncRNAs (long intergenic non-coding RNA) located in the long arm of the chromosome 18. The purpose of this study was to determine the expression of LOC100287225 in colorectal tissue, and its misregulation in CRC patients. Quantitative real-time-PCR (qRT-PCR) was used to investigate the LOC100287225 expression in pairs of tumorous and adjacent tumor-free tissues of 39 colorectal cancer patients. Also, the relationship between the clinicopathology and expression of LOC100287225 was determined. QRT-PCR results revealed that not only is LOC100287225 expressed in the intestinal tissue, but has also been misregulated during tumorigenesis. Moreover, LOC100287225 RNA relative expression levels were significantly lower in tumor tissues compared with adjacent tumor-free tissues (P< 0.001). RNA expression level of LOC100287225 did not show significant correlation with clinical characteristics. In conclusion, our study demonstrated that LOC100287225 misregulation could be a potential target for gene therapy in colorectal cancer.
Insights
This study found that LOC100287225, a long non-coding RNA, is downregulated in colorectal cancer (CRC) tissues. This misregulation suggests LOC100287225 may be a potential therapeutic target for CRC.
Area of Science:
- Molecular Biology
- Oncology
- Genetics
Background:
- Colorectal cancer (CRC) is a prevalent malignancy necessitating novel therapeutic targets and biomarkers.
- Long non-coding RNAs (lncRNAs) are implicated in various cancers, including CRC, but their specific roles and mechanisms remain largely unelucidated.
- LOC100287225, a long intergenic non-coding RNA on chromosome 18, is a novel lncRNA requiring further investigation.
Purpose of the Study:
- To investigate the expression profile of LOC100287225 in colorectal tissues.
- To determine the potential misregulation of LOC100287225 in colorectal cancer (CRC) patients.
- To explore the correlation between LOC100287225 expression and clinicopathological features in CRC.
Main Methods:
- Quantitative real-time-PCR (qRT-PCR) was employed to assess LOC100287225 expression levels.
- Paired tumorous and adjacent tumor-free colorectal tissues from 39 CRC patients were analyzed.
- Statistical analyses were performed to correlate gene expression with clinicopathological data.
Main Results:
- LOC100287225 is expressed in intestinal tissues and exhibits significant misregulation during colorectal tumorigenesis.
- Relative expression levels of LOC100287225 were markedly lower in tumor tissues compared to adjacent non-tumor tissues (P<0.001).
- No significant correlation was observed between LOC100287225 RNA expression levels and the clinical characteristics of CRC patients.
Conclusions:
- The study demonstrates that LOC100287225 is downregulated in colorectal cancer tissues.
- Misregulation of LOC100287225 presents a potential biomarker for CRC.
- LOC100287225 warrants further investigation as a potential target for gene therapy in colorectal cancer treatment.
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