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Updated: Mar 22, 2026

Metabolic Profile Analysis of Zebrafish Embryos
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Measuring embryo metabolism to predict embryo quality.

Jeremy G Thompson1, Hannah M Brown1, Melanie L Sutton-McDowall1

  • 1ARC Centre of Excellence for Nanoscale BioPhotonics, Robinson Research Institute, School of Medicine, The University of Adelaide, Adelaide, SA 5005, Australia.

Reproduction, Fertility, and Development
|April 12, 2016
PubMed
Summary

Measuring early embryo metabolism can predict development for better assisted reproduction technology (ART) outcomes and livestock breeding. Current methods are limited, but new autofluorescence imaging shows promise for assessing embryo quality.

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Area of Science:

  • Reproductive Biology
  • Biotechnology
  • Metabolic Engineering

Background:

  • Embryo metabolism is a key indicator of developmental potential.
  • Current assisted reproduction technology (ART) and livestock breeding lack precise embryo viability markers.
  • Existing metabolic assessment methods have not achieved widespread clinical or production use.

Purpose of the Study:

  • To review existing techniques for measuring early embryo metabolism.
  • To evaluate the suitability of these techniques for human ART and livestock applications.
  • To introduce and advocate for multi-spectral analysis as a novel embryo quality assessment method.

Main Methods:

  • Review of indirect techniques measuring the embryo's microenvironment.
  • Review of direct techniques measuring intracellular metabolic activity.

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Last Updated: Mar 22, 2026

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  • Introduction of multi- (or hyper-) spectral analysis using autofluorescence.

    • Main Results:

    • Both indirect and direct metabolic measurement techniques have limitations for clinical use.
    • Direct intracellular measurements may be suitable for livestock applications.
    • Multi-spectral analysis of autofluorescence presents a promising new avenue for embryo quality assessment.

    Conclusions:

    • Accurate assessment of early embryo metabolism is crucial for improving ART and livestock breeding efficiency.
    • Existing methods face challenges in clinical and production settings.
    • Autofluorescence-based multi-spectral analysis offers a viable alternative for embryo quality evaluation.