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Related Concept Videos

Proteomics01:33

Proteomics

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A proteome is the entire set of proteins that a cell type produces. We can study proteomes using the knowledge of genomes because genes code for mRNAs, and the mRNAs encode proteins. Although mRNA analysis is a step in the right direction, not all mRNAs are translated into proteins.
Proteomics is the study of proteomes' function. It involves the large-scale systematic study of the proteome to denote the protein complement expressed by a genome. Scientist Mark Wilkins coined the term...
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Peptide Identification Using Tandem Mass Spectrometry01:33

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Tandem mass spectrometry, also known as MS/MS or MS2, is an analytical technique that employs two mass analyzers. Essentially it is a series of mass spectrometers that helps isolate a particular biomolecule and then helps study its chemical properties.
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Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
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Related Experiment Video

Updated: Mar 22, 2026

A Spin-Tip Enrichment Strategy for Simultaneous Analysis of N-Glycopeptides and Phosphopeptides from Human Pancreatic Tissues
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Simple and Integrated Spintip-Based Technology Applied for Deep Proteome Profiling.

Wendong Chen1, Shuai Wang2, Subash Adhikari

  • 1Department of Chemistry, Fudan University , Shanghai 200433, China.

Analytical Chemistry
|April 12, 2016
PubMed
Summary
This summary is machine-generated.

A novel integrated spintip proteomics technology (SISPROT) simplifies sample preparation for high-sensitivity mass spectrometry. This method enables efficient protein identification and deep proteome profiling, even with limited cell numbers.

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Area of Science:

  • Proteomics
  • Analytical Chemistry
  • Biotechnology

Background:

  • Mass spectrometry (MS)-based proteomics requires efficient sample preparation for high sensitivity.
  • Existing methods often involve multiple complex steps, leading to potential sample loss.

Purpose of the Study:

  • To develop a simple, integrated spintip-based proteomics technology (SISPROT) for streamlined sample preparation.
  • To enhance sensitivity and efficiency in proteomic analysis, particularly for limited cell samples.

Main Methods:

  • Developed SISPROT, integrating strong cation exchange beads and C18 disk in a single pipet tip.
  • Incorporated protein preconcentration, reduction, alkylation, digestion, desalting, and peptide fractionation within the SISPROT tip.
  • Utilized standard centrifugation for multiplexing and reproducibility.

Main Results:

  • SISPROT achieved high sensitivity with negligible sample loss, identifying 1270 proteins from 2000 cells in 1.4 h MS time.
  • Analysis of 100,000 cells identified 7826 proteins within 22 h MS time.
  • Reproducible multiplexed analysis (Pearson correlation coefficient > 0.98) was demonstrated, including deep proteome profiling of dental stem cells (9078 proteins identified).

Conclusions:

  • SISPROT offers a user-friendly, highly sensitive, and reproducible method for proteomic sample preparation.
  • The technology is well-suited for deep proteome profiling of low cell numbers (<100,000 cells).
  • SISPROT is applicable to translational studies requiring multiplexed, label-free quantification.