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Gene transcription is regulated by the synergistic action of several proteins that form a complex at a gene regulatory site. This is observed in eukaryotes, where the regulation of gene expression is a complex process. Regulatory proteins in eukaryotes can broadly be classified into two types – regulators that bind directly to specific DNA sequences and co-regulators that associate with regulatory proteins but cannot directly bind to the DNA. These co-regulators are further divided into...

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Repressing Gene Transcription by Redirecting Cellular Machinery with Chemical Epigenetic Modifiers

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Published on: September 20, 2018

Transcriptional Regulators Compete with Nucleosomes Post-replication.

Srinivas Ramachandran¹, Steven Henikoff¹

¹Basic Sciences Division, Fred Hutchinson Cancer Research Center, Seattle, WA 98109, USA; Howard Hughes Medical Institute, Seattle, WA 98109, USA.

|April 12, 2016

Summary

This summary is machine-generated.

Chromatin organization is restored after DNA replication through the action of transcription factors, RNA polymerase II, and remodelers. This study reveals how nucleosome landscapes re-emerge minutes post-replication.

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Describing a Transcription Factor Dependent Regulation of the MicroRNA Transcriptome

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Published on: June 15, 2016

Area of Science:

Molecular Biology
Genetics
Epigenetics

Background:

DNA replication requires nucleosome disruption and reformation.
The mechanisms of chromatin re-establishment post-replication remain unclear.

Purpose of the Study:

To investigate how genome-wide chromatin organization is re-established after DNA replication.
To characterize nucleosome and protein locations behind replication forks.

Main Methods:

Developed Mapping In vivo Nascent Chromatin with EdU and sequencing (MINCE-seq).
MINCE-seq provides high temporal and spatial resolution of chromatin post-replication.

Main Results:

Replication disrupts characteristic chromatin landscapes at Drosophila promoters and enhancers.
High RNAPII stalling and DNA accessibility at promoters correlate with BRM remodeler enrichment.
Enhancer chromatin disruption suggests TF competition in nucleosome re-establishment.

Conclusions:

The study elucidates the rapid re-emergence of nucleosome landscapes post-replication.
Transcription factors, RNAPII, and remodelers are key players in restoring chromatin organization.
A uniformly packaged genome transitions to a characteristic landscape minutes after replication.