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A simple method for the characterization of photoproducts from DNA applied to the cis-syn thymine dimer
1Department of Chemistry and Applied Chemistry, University of Natal, Durban, Republic of South Africa.
Biomedical Chromatography : BMC
|January 1, 1989
Abstract:
A method for the unambiguous characterization of DNA photoproducts has been developed. It does not require radio-labelled DNA, or specialized techniques. In the preliminary step, UV-irradiated DNA is hydrolysed to its constituent bases and photoproducts. The photoproducts are then separated from nucleic acid bases using low pressure ion-exchange chromatography on a Dowex 1 x 8 200-400, and an Amberlite CG-50-H+ column. Further separation and purification of photoproducts is carried out on the HPLC Whatman Partisil ODS2 column, using 15% MeOH. The procedure is simple, reproducible and versatile.