Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

In-situ Hybridization

In-situ Hybridization

In situ hybridization (ISH) is a technique used to detect and localize specific DNA or RNA molecules in cells, tissue, or tissue sections using a labeled probe. The technique was first used in 1969 for the investigation of nucleic acids. It is currently an essential tool in scientific research and clinical settings, especially for diagnostic purposes.
Types of probes and labels
A probe is a complementary strand of DNA or RNA that binds to corresponding nucleotide sequences in a cell. Many...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Osteocyte-derived erythroferrone regulates liver hepcidin during stress erythropoiesis.

Blood advances·2026

Same author

The ventral CA2 region of the hippocampus and its differential contributions to social memory and social aggression.

Cell reports·2025

Same author

Osteocyte-derived erythroferrone regulates liver hepcidin during stress erythropoiesis.

bioRxiv : the preprint server for biology·2025

Same author

Management of malignant pleural effusions and drainage care: a hospital in the home and palliative care service.

Internal medicine journal·2024

Same author

The hippocampal CA2 region discriminates social threat from social safety.

Nature neuroscience·2024

Same author

G protein coupled receptor transcripts in human immune cells and platelets.

Scientific data·2024

Search research articles

Related Experiment Video

Updated: Mar 22, 2026

Combining Multiplex Fluorescence In Situ Hybridization with Fluorescent Immunohistochemistry on Fresh Frozen or Fixed Mouse Brain Sections

Combining Multiplex Fluorescence In Situ Hybridization with Fluorescent Immunohistochemistry on Fresh Frozen or Fixed Mouse Brain Sections

Published on: June 25, 2021

Hybridization Histochemistry of Neural Transcripts.

W Scott Young¹, June Song¹, Éva Mezey²

¹Section on Neural Gene Expression, National Institute of Mental Health, National Institutes of Health, Bethesda, Maryland.

Current Protocols in Neuroscience

|April 12, 2016

Summary

In situ hybridization enables precise localization and quantification of RNA transcripts within cells. This technique uses various probes, including oligodeoxynucleotides and riboprobes, for detailed gene expression analysis.

More Related Videos

Radioactive in situ Hybridization for Detecting Diverse Gene Expression Patterns in Tissue

Radioactive in situ Hybridization for Detecting Diverse Gene Expression Patterns in Tissue

Published on: April 27, 2012

Combining Double Fluorescence In Situ Hybridization with Immunolabelling for Detection of the Expression of Three Genes in Mouse Brain Sections

Combining Double Fluorescence In Situ Hybridization with Immunolabelling for Detection of the Expression of Three Genes in Mouse Brain Sections

Published on: March 26, 2016

Related Experiment Videos

Last Updated: Mar 22, 2026

Combining Multiplex Fluorescence In Situ Hybridization with Fluorescent Immunohistochemistry on Fresh Frozen or Fixed Mouse Brain Sections

Combining Multiplex Fluorescence In Situ Hybridization with Fluorescent Immunohistochemistry on Fresh Frozen or Fixed Mouse Brain Sections

Published on: June 25, 2021

Radioactive in situ Hybridization for Detecting Diverse Gene Expression Patterns in Tissue

Radioactive in situ Hybridization for Detecting Diverse Gene Expression Patterns in Tissue

Published on: April 27, 2012

Combining Double Fluorescence In Situ Hybridization with Immunolabelling for Detection of the Expression of Three Genes in Mouse Brain Sections

Combining Double Fluorescence In Situ Hybridization with Immunolabelling for Detection of the Expression of Three Genes in Mouse Brain Sections

Published on: March 26, 2016

Area of Science:

Molecular Biology
Cell Biology
Genetics

Background:

Gene expression is fundamentally linked to RNA transcript production within cells.
Hybridization histochemistry, also known as in situ hybridization, offers cellular-level resolution for localizing these transcripts.
Quantification of transcript levels across different tissues or physiological states is crucial for biological insights.

Purpose of the Study:

To describe a comprehensive set of hybridization histochemical techniques for RNA transcript localization and quantification.
To detail methods utilizing both oligodeoxynucleotide and RNA (riboprobe) probes.
To present protocols for various detection systems and probe preparation.

Main Methods:

Utilized oligodeoxynucleotide probes (Basic Protocols 1, 2, Alternate Protocol 1) and RNA probes (riboprobes; Basic Protocols 3, 5).
Incorporated colorimetric and fluorescent detection methods, including commercial oligodeoxynucleotide sets (Basic Protocol 2).
Described tyramide signal amplification (TSA) and autoradiographic detection, alongside probe labeling and verification (Support Protocols).

Main Results:

Established protocols for in situ hybridization with diverse probe types and detection systems.
Demonstrated the capability for high-resolution localization and quantification of RNA transcripts.
Provided methods for specific applications like Y chromosome detection.

Conclusions:

Hybridization histochemistry is a versatile and powerful technique for studying gene expression at the cellular level.
The described methods offer flexibility in probe choice, detection sensitivity, and application scope.
These protocols facilitate detailed analysis of RNA distribution and abundance in biological samples.