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Related Experiment Videos

Monotonic versus oscillating microtubule assembly: a cryo-electron microscope study.

R H Wade1, F Pirollet, R L Margolis

  • 1Département de Recherche Fondamentale, Centre d'Etudes Nucléaires, Grenoble, France.

Biology of the Cell
|January 1, 1989
PubMed
Summary

Microtubule assembly can be monotonic or oscillatory. Oscillatory assembly involves more inactive tubulin oligomers, suggesting oligomer opening is key to microtubule oscillations.

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Area of Science:

  • Biochemistry
  • Cell Biology
  • Structural Biology

Background:

  • Microtubule dynamics are crucial for cellular processes.
  • Microtubule assembly can proceed via monotonic or oscillatory pathways, influenced by GTP concentration.

Purpose of the Study:

  • To compare tubulin assemblies in monotonic and oscillatory microtubule polymerization.
  • To elucidate the structural differences underlying these two assembly modes.

Main Methods:

  • Cryoelectron microscopy was employed to visualize tubulin assemblies.
  • Analysis focused on the initial phase of microtubule polymerization (first assembly peak).

Main Results:

  • Both monotonic and oscillatory systems showed similar polymerization extent and microtubule length distribution at the first assembly peak.

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  • The oscillatory system exhibited significantly higher quantities of closed ring-like tubulin oligomers compared to the monotonic system.
  • Addition of GTP to the oscillating system, converting it to monotonic, markedly reduced the number of observed rings.

    • Conclusions:

    • The GTP-dependent step governing microtubule oscillations is proposed to be the opening of inactive tubulin oligomers.
    • Ring-like tubulin oligomers may represent a key intermediate in the oscillatory polymerization pathway.