Jove
Visualize
Contact Us

Related Concept Videos

Cryo-electron Microscopy01:28

Cryo-electron Microscopy

4.5K
Conventional electron microscopy (EM) involves dehydration, fixation, and staining of biological samples, which distorts the native state of biological molecules and results in several artifacts. Also, the high-energy electron beam damages the sample and makes it difficult to obtain high-resolution images. These issues can be addressed using cryo-EM, which uses frozen samples and gentler electron beams. The technique was developed by Jacques Dubochet, Joachim Frank, and Richard Henderson, for...
4.5K
Preparation of Samples for Electron Microscopy01:20

Preparation of Samples for Electron Microscopy

7.7K
To be visualized by an electron microscope, either transmission or scanning, biological samples need to be fixed (stabilized) so the electron beam does not destroy them and dried thoroughly (desiccated/dehydrated) so the vacuum does not affect them. Fixation needs to be done as quickly as possible because the sample properties will start changing as soon as it is removed from its natural environment. For example, in a tissue sample, the oxygen levels begin decreasing, causing an altered...
7.7K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Near-equilibrium isotope fractionation during planetesimal evaporation.

Icarus·2019
Same author

A single sub-kilometre Kuiper belt object from a stellar occultation in archival data.

Nature·2009
Same author

Ion transport across CF and normal murine olfactory and ciliated epithelium.

American journal of physiology. Cell physiology·2009
Same author

Expression of CFTR from a ciliated cell-specific promoter is ineffective at correcting nasal potential difference in CF mice.

Gene therapy·2007
Same author

Culture of murine nasal epithelia: model for cystic fibrosis.

American journal of physiology. Lung cellular and molecular physiology·2005
Same author

Aflatoxicosis in turkey poults is prevented by treatment of naturally contaminated corn with ozone generated by electrolysis.

Poultry science·1998
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Video

Updated: Mar 22, 2026

Visualization of DNA Compaction in Cyanobacteria by High-voltage Cryo-electron Tomography
09:47

Visualization of DNA Compaction in Cyanobacteria by High-voltage Cryo-electron Tomography

Published on: July 17, 2018

9.8K

FREEZE-DRYING OF BLUE-GREEN ALGAE FOR ELECTRON MICROSCOPE STUDIES1.

T D Rogers1, V E Scholes1, H E Schlichting1

  • 1Department of Biology, North Texas State University, Denton. Texas.

Journal of Phycology
|April 12, 2016
PubMed
Summary
This summary is machine-generated.

Freeze-drying cyanobacteria, including Anabaena cylindrica, improves electron microscopy by making photosynthetic thylakoids clearer. This method also revealed a distinct mucous capsule in Eucapsis sp. cells.

More Related Videos

Plunge Freezing: A Tool for the Ultrastructural and Immunolocalization Studies of Suspension Cells in Transmission Electron Microscopy
13:35

Plunge Freezing: A Tool for the Ultrastructural and Immunolocalization Studies of Suspension Cells in Transmission Electron Microscopy

Published on: May 5, 2017

11.6K
Preparation of Prokaryotic and Eukaryotic Organisms Using Chemical Drying for Morphological Analysis in Scanning Electron Microscopy SEM
09:58

Preparation of Prokaryotic and Eukaryotic Organisms Using Chemical Drying for Morphological Analysis in Scanning Electron Microscopy SEM

Published on: January 7, 2019

20.2K

Related Experiment Videos

Last Updated: Mar 22, 2026

Visualization of DNA Compaction in Cyanobacteria by High-voltage Cryo-electron Tomography
09:47

Visualization of DNA Compaction in Cyanobacteria by High-voltage Cryo-electron Tomography

Published on: July 17, 2018

9.8K
Plunge Freezing: A Tool for the Ultrastructural and Immunolocalization Studies of Suspension Cells in Transmission Electron Microscopy
13:35

Plunge Freezing: A Tool for the Ultrastructural and Immunolocalization Studies of Suspension Cells in Transmission Electron Microscopy

Published on: May 5, 2017

11.6K
Preparation of Prokaryotic and Eukaryotic Organisms Using Chemical Drying for Morphological Analysis in Scanning Electron Microscopy SEM
09:58

Preparation of Prokaryotic and Eukaryotic Organisms Using Chemical Drying for Morphological Analysis in Scanning Electron Microscopy SEM

Published on: January 7, 2019

20.2K

Area of Science:

  • Microbiology
  • Electron Microscopy
  • Cyanobacteria Research

Background:

  • Accurate ultrastructural preservation is crucial for understanding microbial cell biology.
  • Traditional fixation methods can introduce artifacts, potentially obscuring fine cellular details.

Purpose of the Study:

  • To evaluate the efficacy of freeze-drying as a preparation technique for electron microscopy of cyanobacteria.
  • To compare the ultrastructural preservation of freeze-dried Anabaena cylindrica and Eucapsis sp. with conventionally prepared specimens.

Main Methods:

  • Cultures of Anabaena cylindrica and Eucapsis sp. were harvested.
  • Specimens were prepared using freeze-drying and compared to control samples not subjected to freeze-drying.
  • Standard Ryter and Kellenberger fixation and embedding techniques were employed for electron microscopy.

Main Results:

  • Freeze-dried Anabaena cylindrica cells displayed more distinct and less distorted photosynthetic thylakoids compared to control cells.
  • A prominent mucous capsule was observed in freeze-dried Eucapsis sp. cells, which was not discernible in non-freeze-dried controls.
  • Freeze-drying enhanced the visibility of specific ultrastructural features in both cyanobacterial species.

Conclusions:

  • Freeze-drying is a valuable preparation technique for electron microscopy, offering superior preservation of cyanobacterial ultrastructure.
  • This method aids in the visualization of delicate structures like photosynthetic thylakoids and extracellular capsules.
  • The findings suggest freeze-drying can reveal cellular features not apparent with conventional preparation methods.