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Continuous biohydrogen production from waste bread by anaerobic sludge
Wei Han1, Jingang Huang1, Hongting Zhao1
1College of Materials and Environmental Engineering, Hangzhou Dianzi University, Hangzhou 310018, China.
Bioresource Technology
|April 12, 2016
Summary
This study demonstrates continuous biohydrogen production from waste bread using anaerobic sludge. Optimized hydrolysis and continuous stirred-tank reactor (CSTR) processes achieved efficient hydrogen generation from bread waste.
Area of Science:
- Biotechnology
- Waste Management
- Renewable Energy
Background:
- Waste bread represents a significant organic waste stream with potential for valorization.
- Biohydrogen production offers a sustainable alternative to fossil fuels.
- Previous studies have explored biohydrogen production, but continuous processes from waste bread are underexplored.
Purpose of the Study:
- To investigate continuous biohydrogen production from waste bread using anaerobic sludge.
- To optimize the hydrolysis of waste bread for enhanced glucose yield.
- To determine optimal conditions for biohydrogen generation in a continuous stirred-tank reactor (CSTR).
Main Methods:
- Solid-state fermentation of Aspergillus awamori and Aspergillus oryzae to generate crude enzymes for waste bread hydrolysis.
- Hydrolysis of waste bread at a 15% (w/v) ratio to produce glucose and free amino nitrogen.
- Continuous biohydrogen production using anaerobic sludge in a CSTR, optimizing for chemical oxygen demand (COD).
Main Results:
- Hydrolysis yielded 49.78 g/L glucose and 284.12 mg/L free amino nitrogen from 15% (w/v) waste bread.
- Optimal hydrogen production rate of 7.4 L/(Ld) was achieved at a COD of 6000 mg/L.
- 1g of waste bread generated 0.332g glucose, yielding 109.5 mL of hydrogen.
Conclusions:
- Continuous biohydrogen production from waste bread is feasible and efficient.
- The developed method offers a sustainable route for waste bread valorization.
- This study presents the first report on continuous biohydrogen production from waste bread using anaerobic sludge.
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