Positive effects of biostimulation on luteinizing hormone concentration and follicular development in anestrous beef...

The objectives of this study were to characterize the LH secretion pattern and the follicular development of anestrous beef heifers during early exposure (first 30 d of exposure) to androgenized steers (AS) and to determine if exposure to AS for 80 d (includes the first 30 d and 50 d more) advances the onset of ovarian cyclic activity. Twenty-nine anestrous Hereford heifers (20.2 ± 4.1 mo old and 257.5 ± 32.5 kg of BW) were allocated to 2 homogeneous groups according to their age and BW: 1) heifers exposed to AS (group EH; = 15) for 80 d and 2) control heifers, isolated from AS and any other male during all the course of the study (group CH; = 14). On d 0, 3 AS were joined with the EH group, which were removed and replaced with other 3 AS on Day 14. On d -10, 1, 10, 20, and 30, 8 heifers per group were housed in individual stalls and blood samples for LH were collected at 15-min intervals for 6 h. From d -10 to 30, the maximum follicle diameter (MFD) and the presence of a corpus luteum (CL) was daily recorded by ultrasound scanning and estrous behavior was detected twice daily. The emergence of follicular waves (FW), defined as the day when the dominant follicle of a wave was first observed (3-4 mm diam.), was retrospectively determined. Afterward, ultrasound scannings were performed weekly from d 32 to 60 and on d 70 and 80 to determine the presence of CL. After 10 d of male exposure, LH concentrations, either mean (1.67 vs. 0.88 ng/mL [SEM 0.09]) or basal (1.53 vs. 0.74 ng/mL [SEM 0.09]), were greater ( < 0.05) in the EH group than in the CH group. There was a treatment effect in MFD, as it was greater in EH than in CH ( = 0.05; 8.00 ± 0.16 vs. 7.52 ± 0.17 mm, respectively), but none of those follicles ovulated during the 40-d period. The MFD of the second FW was greater in EH than in CH, in coincidence with the transient increase on LH concentrations, which probably induced the greater follicular growth. Cumulative proportions of heifers that started to cycle were greater ( = 0.01) in EH than in CH on d 60 (33.3 vs. 0%), 70 (47 vs. 0%; < 0.005), and 80 (53 vs. 0%; < 0.001) of the exposure period. In conclusion, exposure of anestrous beef heifers to AS resulted in a transient increase on LH secretion after 10 d of male exposure and increased follicular diameter attained during the second FW. In addition, ovarian cyclic activity was advanced in exposed heifers.