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Related Concept Videos

Golgi Apparatus

Golgi Apparatus

As they leave the Endoplasmic Reticulum (ER), properly folded and assembled proteins are selectively packaged into vesicles. These vesicles are transported by microtubule-based motor proteins and fuse together to form vesicular tubular clusters, subsequently arriving at the Golgi apparatus, a eukaryotic endomembrane organelle that often has a distinctive ribbon-like appearance.

Golgi Apparatus

Golgi Apparatus

Properly folded and assembled proteins are selectively packaged into vesicles that exit the ER. Motor proteins transport these vesicles to the Golgi apparatus for adding modifications that make these proteins functional at their destination.
The Golgi apparatus is a eukaryotic organelle that has a distinctive ribbon-like appearance. It is a primary sorting and dispatch station for cargo arriving from the ER. Newly arriving vesicles enter the cis face of the Golgi, closest to the ER, and are...

Golgi Apparatus

Golgi Apparatus

Golgi Matrix Proteins

Golgi Matrix Proteins

Golgi matrix proteins are a group of highly dynamic proteins that maintain the stacked structure of Golgi. These proteins adapt to rapid morphological changes of the Golgi during the cell cycle. During cell division, mild proteolysis removes these connections resulting in Golgi unstacking. In The daughter cells, these proteins help reassemble the unstacked Golgi.
One of the first identified Golgi matrix proteins was GM130, a rod-like protein located in the cis-Golgi. Subsequently, many Golgi...

Transport Across the Golgi

Transport Across the Golgi

While it is unclear how molecules move between adjacent Golgi cisternae, it is apparent that the molecules move from cis- cisterna, the entry face, to the trans- cisterna, the exit face. Experiments initially suggested vesicles that bud from one cisterna and fuse with the next cisterna to transport proteins between the cisternae. This vesicular transport model describes the Golgi apparatus as a relatively static structure with a unique enzyme composition in each cisterna. Molecules are...

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Related Experiment Video

Updated: Mar 22, 2026

Visualizing the Effects of a Positive Early Experience, Tactile Stimulation, on Dendritic Morphology and Synaptic Connectivity with Golgi-Cox Staining

Visualizing the Effects of a Positive Early Experience, Tactile Stimulation, on Dendritic Morphology and Synaptic Connectivity with Golgi-Cox Staining

Published on: September 25, 2013

Golgi-Cox Staining Step by Step.

Sami Zaqout¹, Angela M Kaindl¹

¹Institute of Cell Biology and Neurobiology, Charité - Universitätsmedizin BerlinBerlin, Germany; Center for Chronically Sick Children (Sozialpädiatrisches Zentrum, SPZ), Charité - Universitätsmedizin BerlinBerlin, Germany.

Frontiers in Neuroanatomy

|April 12, 2016

Summary

This summary is machine-generated.

Golgi staining is crucial for studying neuronal morphology. This detailed protocol simplifies the Golgi-Cox staining method, making it easily accessible for neuroscientists and reducing experimental frustration.

Keywords:

Golgi dendrites neuronal morphology spines vibratome

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Assessment of Hippocampal Dendritic Complexity in Aged Mice Using the Golgi-Cox Method

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Related Experiment Videos

Last Updated: Mar 22, 2026

Visualizing the Effects of a Positive Early Experience, Tactile Stimulation, on Dendritic Morphology and Synaptic Connectivity with Golgi-Cox Staining

Visualizing the Effects of a Positive Early Experience, Tactile Stimulation, on Dendritic Morphology and Synaptic Connectivity with Golgi-Cox Staining

Published on: September 25, 2013

Imaging Neurons within Thick Brain Sections Using the Golgi-Cox Method

Imaging Neurons within Thick Brain Sections Using the Golgi-Cox Method

Published on: April 18, 2017

Assessment of Hippocampal Dendritic Complexity in Aged Mice Using the Golgi-Cox Method

Assessment of Hippocampal Dendritic Complexity in Aged Mice Using the Golgi-Cox Method

Published on: June 22, 2017

Area of Science:

Neuroscience
Cell Biology
Histology

Background:

Golgi staining is essential for visualizing neuronal structure.
Existing Golgi staining protocols often lack critical procedural details.
This can lead to difficulties and delays in laboratory implementation.

Purpose of the Study:

To provide a highly detailed and easily reproducible Golgi-Cox staining protocol.
To simplify the establishment of Golgi staining in neuroscience research laboratories.
To enhance the accessibility of neuronal morphology studies.

Main Methods:

Detailed description of the Golgi-Cox staining procedure.
Emphasis on critical steps and potential pitfalls.
Standardized protocol for consistent results.

Main Results:

The described protocol significantly simplifies Golgi-Cox staining.
The method is shown to be easily feasible for researchers.
Successful application of the detailed protocol for neuronal morphology analysis.

Conclusions:

This detailed Golgi-Cox staining protocol facilitates neuronal morphology studies.
The method is readily implementable in diverse neuroscience labs.
It aims to reduce the time and frustration associated with establishing Golgi staining.