Related Experiment Video
Updated: Mar 22, 2026
08:39
Author Spotlight: Polysome Profiling Protocol for Studying Translational Regulation in Arabidopsis Under Heat Stress
Published on: October 11, 2024
2.3K
Transcriptome Response Mediated by Cold Stress in Lotus japonicus
Pablo I Calzadilla1, Santiago J Maiale1, Oscar A Ruiz1
1Unidad de Biotecnología 1, Instituto de Investigaciones Biotecnológicas, Instituto Tecnológico de Chascomús, Universidad Nacional de San Martín, Consejo Nacional de Investigaciones Científicas y Técnicas Chascomús, Argentina.
Frontiers in Plant Science
|April 12, 2016
Summary
This study reveals how Lotus japonicus responds to cold stress, identifying key genes involved in acclimation. These findings can help breed forage legumes better suited for cooler climates.
Area of Science:
- Plant Science
- Molecular Biology
- Agricultural Science
Background:
- Lotus species are vital forage crops, valued for nutrition and stress tolerance.
- Their dry matter yield decreases in cooler seasons, with limited research on cold acclimation mechanisms.
Purpose of the Study:
- To investigate the cold acclimation response in the model legume Lotus japonicus.
- To identify genes and transcription factors involved in low-temperature stress tolerance.
Main Methods:
- Utilized high-throughput RNA sequencing to analyze gene expression changes in Lotus japonicus under cold stress.
- Focused on a 24-hour stress period to capture early acclimation responses.
Main Results:
- Identified 1077 differentially expressed genes, with 713 up-regulated (involved in lipid, cell wall, phenylpropanoid, sugar, and proline regulation) and 364 down-regulated (affecting photosynthesis and chloroplast development).
- Discovered 41 cold-inducible transcription factors, including AP2/ERF, NAC, MYB, and WRKY families, with two novel factors identified.
- Characterized the cold stress expression profiles of DREB1/CBF transcription factors.
Conclusions:
- This is the first transcriptome profiling of Lotus japonicus under cold stress, providing a comprehensive molecular overview.
- The identified genes and transcription factors offer valuable targets for breeding forage legumes with enhanced cold acclimation capabilities.
More Related Videos
Related Concept Videos
02:45Responses to Heat and Cold Stress
15.6K
Every organism has an optimum temperature range within which healthy growth and physiological functioning can occur. At the ends of this range, there will be a minimum and maximum temperature that interrupt biological processes.
15.6K
01:10Transcription
158.5K
Overview
Transcription is the process of synthesizing RNA from a DNA sequence by RNA polymerase. It is the first step in producing a protein from a gene sequence. Additionally, many other proteins and regulatory sequences are involved in the proper synthesis of messenger RNA (mRNA). Regulation of transcription is responsible for the differentiation of all the different types of cells and often for the proper cellular response to environmental signals.
Transcription Can Produce Different Kinds...
Transcription is the process of synthesizing RNA from a DNA sequence by RNA polymerase. It is the first step in producing a protein from a gene sequence. Additionally, many other proteins and regulatory sequences are involved in the proper synthesis of messenger RNA (mRNA). Regulation of transcription is responsible for the differentiation of all the different types of cells and often for the proper cellular response to environmental signals.
Transcription Can Produce Different Kinds...
158.5K
02:02Responses to Salt Stress
15.0K
Salt stress—which can be triggered by high salt concentrations in a plant’s environment—can significantly affect plant growth and crop production by influencing photosynthesis and the absorption of water and nutrients.
15.0K
01:30Other Stress Responses in Bacteria
504
Bacteria have global regulatory systems that control several types of stress mechanisms. These include Pho regulon and the heat shock response, which are essential systems for environmental adaptation, such as nutrient limitation and proteotoxic stress. The Pho regulon and the heat shock response exemplify bacterial resilience, enabling rapid adaptation to fluctuating environmental conditions.Pho RegulonBacteria require phosphorus for essential cellular processes, including nucleic acid...
504
01:17Gene Regulation During Sporulation
629
Sporulation is a complex developmental process that allows certain Gram-positive bacteria, such as Bacillus subtilis and Clostridium species, to survive extreme environmental conditions. This process is tightly regulated by a series of signaling cascades and transcriptional controls, ensuring the formation of a highly resistant endospore.Sporulation is triggered by unfavorable conditions, such as nutrient depletion, and is governed by a phosphorelay system. One of the sensor kinases, such as...
629
01:23Stringent Response in E. coli
463
Bacterial growth is closely tied to nutrient availability, with cells proliferating exponentially under favorable conditions and entering a stationary phase when resources become scarce. This transition is mediated by a regulatory mechanism known as the stringent response, which allows bacteria to adapt to nutrient deprivation by modulating gene expression and metabolic activity.During nutrient scarcity, intracellular amino acid levels decline. It results in the accumulation of uncharged tRNAs...
463