Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Delivery Pathways to the Lysosome01:36

Delivery Pathways to the Lysosome

10.5K
Eukaryotic cells use different mechanisms to eliminate toxic waste obsolete and worn-out substances. Lysosomes play a pivotal role in this, and hence, these substances are carried to the lysosome from other parts of the cell and extracellular space through different pathways. The most elaborately studied pathways to the lysosome are the endocytic pathways.
Endocytosis
In endocytosis, the cell membrane takes up macromolecules and particles from the surrounding medium. Clathrin-mediated...
10.5K
Receptor-mediated Endocytosis01:38

Receptor-mediated Endocytosis

113.2K
Overview
113.2K
Receptor-mediated Endocytosis01:20

Receptor-mediated Endocytosis

11.4K
Receptor-mediated endocytosis is when bulk amounts of specific molecules are imported into a cell after binding to cell surface receptors. The molecules bound to these receptors are taken into the cell through inward folding of the cell surface membrane, which is eventually pinched off into a vesicle within the cell. Structural proteins, such as clathrin, coat the budding vesicle.
Clathrin-Mediated Endocytosis of LDL
One well-characterized example of receptor-mediated endocytosis is the...
11.4K
Receptor-Mediated Endocytosis01:20

Receptor-Mediated Endocytosis

5.7K
5.7K
Lysosomes01:31

Lysosomes

27.0K
Lysosomes are membrane-enclosed spherical sacs derived from the Golgi apparatus. The most important function of the lysosome is degrading macromolecules and biological polymers that are released during membrane trafficking events such as the secretory, endocytic, autophagic, and phagocytic pathways. The degradation is carried out by several hydrolytic enzymes active in an acidic environment of the lysosomal lumen. These acid hydrolases are involved in cellular processes such as cell signaling,...
27.0K
Lysosomes01:31

Lysosomes

3.7K
3.7K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Type III intermediate filaments as novel CoAlation targets.

Redox report : communications in free radical research·2026
Same author

Corrigendum to "Alexander disease mutations differentially sensitize glial fibrillary acidic protein (GFAP) to posttranslational modifications and network disruption by oxidants" [Redox Biology 92 (2026) 104103].

Redox biology·2026
Same author

Lysosomal function, resistance to oxidative stress and repair are compromised by expression of the Alexander disease GFAP R239C mutant.

Free radical biology & medicine·2026
Same author

Alexander disease mutations differentially sensitize glial fibrillary acidic protein (GFAP) to posttranslational modifications and network disruption by oxidants.

Redox biology·2026
Same author

Plectin associates with focal adhesions and contributes to cytoskeletal organization and mechanical properties of astrocytes.

American journal of physiology. Cell physiology·2025
Same author

Mutations in GFAP Alter Early Lineage Commitment of Organoids.

Glia·2025

Related Experiment Video

Updated: Mar 22, 2026

Exploring the Regulation of Lipid Droplet Catabolism through Lipophagy
07:20

Exploring the Regulation of Lipid Droplet Catabolism through Lipophagy

Published on: January 31, 2025

1.4K

Taking a lipidation-dependent path toward endolysosomes.

Clara L Oeste1, Marta Martínez-López1, Dolores Pérez-Sala1

  • 1Department of Chemical and Physical Biology; Centro de Investigaciones Biológicas, CSIC ; Madrid, Spain.

Communicative & Integrative Biology
|April 12, 2016
PubMed
Summary
This summary is machine-generated.

A conserved protein sequence (CINCCKVL) marks cellular endolysosomes without altering their structure. This lipidation-dependent targeting is effective across diverse species, offering a new tool for studying late endocytic compartments.

Keywords:
Rho proteinsfluorescent markers/proteinsisoprenylationlysosomes/endolysosomesmultivesicular bodiesorganelle probepalmitoylationsubcellular localization

More Related Videos

Lipid Droplet Isolation for Quantitative Mass Spectrometry Analysis
10:23

Lipid Droplet Isolation for Quantitative Mass Spectrometry Analysis

Published on: April 17, 2017

10.8K
Author Spotlight: Tackling Challenges in Synthetic Cell Engineering
10:56

Author Spotlight: Tackling Challenges in Synthetic Cell Engineering

Published on: April 12, 2024

1.8K

Related Experiment Videos

Last Updated: Mar 22, 2026

Exploring the Regulation of Lipid Droplet Catabolism through Lipophagy
07:20

Exploring the Regulation of Lipid Droplet Catabolism through Lipophagy

Published on: January 31, 2025

1.4K
Lipid Droplet Isolation for Quantitative Mass Spectrometry Analysis
10:23

Lipid Droplet Isolation for Quantitative Mass Spectrometry Analysis

Published on: April 17, 2017

10.8K
Author Spotlight: Tackling Challenges in Synthetic Cell Engineering
10:56

Author Spotlight: Tackling Challenges in Synthetic Cell Engineering

Published on: April 12, 2024

1.8K

Area of Science:

  • Cell Biology
  • Molecular Biology
  • Biochemistry

Background:

  • The C-terminus of human RhoB protein contains isoprenylation and palmitoylation motifs.
  • These motifs facilitate intraluminal vesicle delivery of proteins across species.
  • Existing endolysosomal markers like Lamp1, Rab7, and full-length RhoB can cause cellular artifacts upon overexpression.

Purpose of the Study:

  • To investigate the utility of the CINCCKVL sequence as a novel endolysosomal marker.
  • To determine if CINCCKVL chimeras can mark endolysosomes without disrupting cellular morphology.
  • To assess the conserved nature of lipidation-dependent endolysosomal targeting.

Main Methods:

  • Construction of fluorescent protein chimeras containing the CINCCKVL sequence.
  • Cellular expression of these chimeras in various cell types.
  • Microscopic analysis to observe localization and cellular morphology.
  • Comparison with established endolysosomal markers (Lamp1, Rab7, RhoB).

Main Results:

  • CINCCKVL chimeras undergo isoprenylation and palmitoylation in cells.
  • These modified chimeras specifically localize to endolysosomes, preserving their morphology.
  • Unlike conventional markers, CINCCKVL chimeras do not induce lysosomal enlargement or actin stress fibers.
  • Lipidation-dependent localization of CINCCKVL chimeras is observed across diverse cell types, indicating conserved targeting.

Conclusions:

  • The CINCCKVL sequence, when fused to fluorescent proteins, serves as a robust and artifact-free marker for endolysosomes.
  • Lipidation-dependent targeting to endolysosomes is a conserved mechanism across species.
  • CINCCKVL chimeras provide a valuable tool for studying late endocytic compartments without perturbing cellular processes.