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Related Concept Videos

Next-generation Sequencing03:00

Next-generation Sequencing

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The first human genome sequencing project cost $2.7 billion and was declared complete in 2003, after 15 years of international cooperation and collaboration between several research teams and funding agencies. Today, with the advent of next-generation sequencing technologies, the cost and time of sequencing a human genome have dropped over 100 fold.
Next-Generation Sequencing Methods
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Sanger Sequencing01:57

Sanger Sequencing

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DNA sequencing is a fundamental technique that is routinely used in the biological sciences. This method can be applied to a range of questions at different scales - from the sequencing of a cloned DNA fragment or the study of a mutation in a gene up to whole-genome sequencing. However, despite the widespread use of sequencing today, it was not until 1977 that Fredrick Sanger and his collaborators developed the chain-termination method to decode DNA sequences. It relies on the separation of a...
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Maxam-Gilbert Sequencing01:05

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In the same year as the discovery of the Sanger sequencing method, another group of scientists, Allan Maxam and Walter Gilbert, demonstrated their chemical-cleavage method for DNA sequencing. The Maxam-Gilbert method relies on using different chemicals that can cleave the DNA sequence at specific sites, the separation of resulting DNA fragments of variable size using electrophoresis, and deciphering the DNA sequence from the resulting gel bands.
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RNA-seq03:21

RNA-seq

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RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
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Related Experiment Video

Updated: Mar 22, 2026

Targeted Next-generation Sequencing and Bioinformatics Pipeline to Evaluate Genetic Determinants of Constitutional Disease
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Targeted Next-generation Sequencing and Bioinformatics Pipeline to Evaluate Genetic Determinants of Constitutional Disease

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Next-generation sequencing still needs our generation's clinicians.

A Reghan Foley1, Sandra Donkervoort1, Carsten G Bönnemann1

  • 1Neuromuscular and Neurogenetic Disorders of Childhood Section, Neurogenetics Branch, National Institute of Neurological Disorders and Stroke, NIH, Bethesda, MD.

Neurology. Genetics
|April 12, 2016
PubMed
Summary
This summary is machine-generated.

Next-generation sequencing (NGS) offers a faster, more cost-effective way to analyze multiple genes simultaneously. This advanced genetic sequencing technology is revolutionizing medical practices, particularly in neurology.

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Area of Science:

  • Genomics and Bioinformatics
  • Medical Genetics
  • Neurology

Background:

  • Traditional Sanger sequencing analyzes genes one at a time.
  • The advent of next-generation sequencing (NGS) marks a significant shift in genetic analysis.
  • NGS enables parallel sequencing of numerous genes.

Purpose of the Study:

  • To highlight the transformative impact of NGS in medicine.
  • To contrast NGS with conventional sequencing methods.
  • To underscore the efficiency and cost-effectiveness of NGS.

Main Methods:

  • Massively parallel sequencing approach.
  • High-throughput data generation.
  • Comparative analysis with Sanger sequencing.

Main Results:

  • NGS allows simultaneous capture and sequencing of a large number of genes.
  • It generates substantial genetic data rapidly.
  • NGS significantly reduces the cost per gene sequenced.

Conclusions:

  • NGS is revolutionizing medical practice, especially in neurology.
  • The technology offers a paradigm shift from traditional gene-by-gene analysis.
  • NGS provides a more efficient and economical approach to genetic diagnostics.