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Updated: Mar 22, 2026

Generation of Multicue Cellular Microenvironments by UV-Photopatterning of Three-Dimensional Cell Culture Substrates
Published on: June 2, 2022
From nano to micro: topographical scale and its impact on cell adhesion, morphology and contact guidance
Anh Tuan Nguyen1, Sharvari R Sathe1, Evelyn K F Yim1,2,3,4
1Mechanobiology Institute, National University of Singapore, Singapore.
Abstract:
Topography, among other physical factors such as substrate stiffness and extracellular forces, is known to have a great influence on cell behaviours. Optimization of topographical features, in particular topographical dimensions ranging from nanoscale to microscale, is the key strategy to obtain the best cellular performance for various applications in tissue engineering and regenerative medicine. In this review, we provide a comprehensive survey on the significance of sizes of topography and their impacts on cell adhesion, morphology and alignment, and neurite guidance. Also recent works mimicking the hierarchical structure of natural extracellular matrix by combining both nanoscale and microscale topographies are highlighted.
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