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Dextran Preserves Native Corneal Structure During Decellularization.

Amy P Lynch^1,2, Samantha L Wilson^3,4, Mark Ahearne^1,2

¹1 Trinity Centre for Bioengineering, Trinity Biomedical Sciences Institute , Trinity College Dublin, Dublin, Republic of Ireland .

Tissue Engineering. Part C, Methods

|April 13, 2016

Summary

This summary is machine-generated.

Dextran effectively reduces corneal swelling during decellularization, preserving native tissue structure and biomechanical properties for corneal regeneration scaffolds. This method enhances cell viability on decellularized tissues.

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Area of Science:

Biomaterials Science
Tissue Engineering
Ophthalmology

Background:

Corneal regeneration relies on decellularized scaffolds.
Tissue swelling during decellularization limits scaffold application.
Maintaining native corneal structure is crucial for function.

Purpose of the Study:

Investigate dextran's role in reducing swelling during corneal decellularization.
Assess dextran's impact on preserving corneal ultrastructure and biomechanical properties.
Evaluate the viability of cells on dextran-treated decellularized corneal scaffolds.

Main Methods:

Corneal decellularization using Triton X-100, sodium dodecyl sulfate, and nucleases.
Supplementation of decellularization solutions with 5% dextran.
Quantitative DNA analysis, transmission electron microscopy (TEM), anisotropy analysis, and compressive modulus testing.

Main Results:

Dextran significantly reduced corneal swelling, maintaining dimensions similar to native tissue.
DNA content was reduced by 96% regardless of dextran presence.
TEM analysis showed native corneal architecture preservation when dextran was present throughout the protocol.
Dextran maintained native-like biomechanical properties and anisotropy.

Conclusions:

Dextran is effective in preventing significant corneal swelling during decellularization.
Dextran enhances the preservation of native corneal ultrastructure and biomechanical integrity.
Dextran-treated decellularized corneal scaffolds support cell viability, making them promising for corneal regeneration.