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Related Concept Videos

Cholesterol: Significance and Regulation01:29

Cholesterol: Significance and Regulation

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Although not a source of energy, cholesterol plays a significant role as a foundational structure for bile salts, steroid hormones, and vitamin D, as well as being a crucial component of plasma membranes. Approximately 15% of blood cholesterol is derived from our diet, with the remainder synthesized from acetyl CoA by the liver and intestines. Cholesterol is eliminated from the body through its conversion into bile salts, which are eventually discarded in the feces.
Considering cholesterol and...
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Peroxisomes01:24

Peroxisomes

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Peroxisomes are specialized organelles present in fungi, plant, and animal cells. It can vary in number, size, morphology, and activity depending on the type of tissue and the nutritional state of the cell. For example, cells with active lipid metabolism, such as adipocytes, neurons, and hepatocytes, have more peroxisomes than other cells in the body. Besides their primary role in breaking down complex organic molecules, peroxisomes can also synthesize specific macromolecules and participate in...
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Peroxisomes01:24

Peroxisomes

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Lipid-derived Compounds in the Human Body01:31

Lipid-derived Compounds in the Human Body

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Fats and lipids are crucial components in the human body. Some lipid-derived compounds, such as fat-soluble vitamins, eicosanoids, lipoproteins, and glycolipids, also play unique roles to support various  biological processes .
Fat-soluble Vitamins
Fat-soluble vitamins, including vitamins A, D, E, and K, are required in minimal quantities, but their deficiencies can lead to severely abnormal physiological conditions. For example, vitamin A deficiency can cause night blindness, dry skin,...
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Overview of Fatty Acid Metabolism01:28

Overview of Fatty Acid Metabolism

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Lipids also are sources of energy that power cellular processes. Like carbohydrates, lipids are composed of carbon, hydrogen, and oxygen, but these atoms are arranged differently. Most lipids are nonpolar and hydrophobic. Major types include fats and oils, waxes, phospholipids, and steroids.
Fatty acids are catabolized in a process called beta-oxidation, which takes place in the matrix of the mitochondria and converts their fatty acid chains into two-carbon units of acetyl groups. The acetyl...
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Oxidation of Alkenes: Syn Dihydroxylation with Osmium Tetraoxide02:44

Oxidation of Alkenes: Syn Dihydroxylation with Osmium Tetraoxide

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Alkenes are converted to 1,2-diols or glycols through a process called dihydroxylation. It involves the addition of two hydroxyl groups across the double bond with two different stereochemical approaches, namely anti and syn. Dihydroxylation using osmium tetroxide progresses with syn stereochemistry.
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Related Experiment Video

Updated: Mar 22, 2026

High-throughput Nitrobenzoxadiazole-labeled Cholesterol Efflux Assay
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High-throughput Nitrobenzoxadiazole-labeled Cholesterol Efflux Assay

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Current trends in oxysterol research.

William J Griffiths1, Jonas Abdel-Khalik2, Thomas Hearn2

  • 1College of Medicine, Grove Building, Swansea University, Singleton Park, Swansea SA2 8PP, U.K. w.j.griffiths@swansea.ac.uk y.wang@swansea.ac.uk.

Biochemical Society Transactions
|April 13, 2016
PubMed
Summary
This summary is machine-generated.

Oxysterols are crucial for brain health, immune responses, and breast cancer. Oxysterol analysis aids in disease diagnosis, offering new research avenues.

Keywords:
RAR-related orphan receptor gamma t (RORγ)cholestenoic acidcholesterolhydroxycholesterolliver X receptor (LXR)sterol regulatory-element binding protein (SREBP)

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Enrichment of Mammalian Tissues and Xenopus Oocytes with Cholesterol
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Enrichment of Mammalian Tissues and Xenopus Oocytes with Cholesterol

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Related Experiment Videos

Last Updated: Mar 22, 2026

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Cell-free Biochemical Fluorometric Enzymatic Assay for High-throughput Measurement of Lipid Peroxidation in High Density Lipoprotein
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Enrichment of Mammalian Tissues and Xenopus Oocytes with Cholesterol
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Area of Science:

  • Biochemistry
  • Immunology
  • Oncology
  • Neuroscience

Background:

  • Oxysterols, cholesterol oxidation products, play multifaceted biological roles.
  • Recent research has illuminated novel functions of oxysterols in various physiological and pathological processes.

Purpose of the Study:

  • To review recent advancements in oxysterol research.
  • To highlight key areas of interest including neuroscience, immunology, and oncology.
  • To discuss the diagnostic potential of oxysterol analysis.

Main Methods:

  • Literature review of recent oxysterol research.
  • Synopsis of studies on oxysterol involvement in neuronal development, immune function, and breast cancer.
  • Discussion of oxysterol analysis techniques for disease diagnosis.

Main Results:

  • Oxysterols are implicated in neuronal development and survival.
  • Oxysterols modulate immune responses, including bacterial/viral infections and Th17-cell differentiation.
  • Oxysterols play a significant role in breast cancer development and progression.

Conclusions:

  • Oxysterol research is a dynamic field with broad implications.
  • Oxysterols are key players in fundamental biological processes and disease pathogenesis.
  • Oxysterol analysis holds promise for improved disease diagnostics.