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The Bone Morphogenetic Protein 15 Up-Regulates the Anti-Müllerian Hormone Receptor Expression in Granulosa Cells
Alice Pierre1, Anthony Estienne1, Chrystèle Racine1
1Université Paris Diderot (A.P., C.R., J.-Y.P., R.F., N.d.C.), Sorbonne Paris Cité, F-75013 Paris, France; Institut National de la Santé et de la Recherche Médicale (A.P., C.R., J.-Y.P., R.F., N.d.C.), Unité 1133, F-75013 Paris, France; Centre National de la Recherche Scientifique (CNRS) (A.P., C.R., J.-Y.P., R.F., N.d.C.), Unité Mixte de Recherche (UMR) 8251, F-75013 Paris, France; Institut National de la Recherche Agronomique (INRA) (A.E., P.J., D.M.), UMR85, F-37380 Nouzilly, France; CNRS (A.E., P.J., D.M.), UMR7247, F-37380 Nouzilly, France; Université François Rabelais de Tours (A.E., P.J., D.M.), F-37041 Tours, France; Institut Français du Cheval et de l'Equitation (A.E., P.J., D.M.), F-37380 Nouzilly, France; Unidad de Producción y Sanidad Animal (B.L., J.L.A., J.F.), Centro de Investigación y Tecnología Agroalimentaria de Aragón (CITA), Instituto Agroalimentario de Aragón - IA2 (CITA-Universidad de Zaragoza), 50059 Zaragoza, España; INRA (S.F.), UMR1388 Génétique, Physiologie et Systèmes d'Elevage (GenPhySE), F-31326 Castanet-Tolosan, France; Université de Toulouse (S.F.), Institut National Polytechnique (INP), École nationale vétérinaire de Toulouse, GenPhySE, F-31076 Toulouse, France; and Université de Toulouse (S.F.), INP, École nationale supérieure agronomique de Toulouse, GenPhySE, F-31326 Castanet-Tolosan, France.
Context:
Anti-Müllerian hormone (AMH) is produced by the granulosa cells (GCs) of growing follicles and inhibits follicular development.
Objective:
This study aimed to investigate the regulation of the AMH-specific type 2 receptor (AMHR2) gene expression in GCs by bone morphogenetic protein (BMP)15, BMP4 and growth differentiation factor (GDF)9.
Design, Setting, And Patients:
Their effects on AMHR2 and AMH mRNAs were studied in luteinized human GCs and in ovine GCs (oGCs) from small antral follicles. The effects of BMPs on human AMHR2 and AMH promoter reporter activities were analyzed in transfected oGCs. The in vivo effect of BMP15 on GCs AMHR2 and AMH expression was investigated by using Lacaune and Rasa Aragonesa hyperprolific ewes carrying loss-of-function mutations in BMP15.
Main Outcome Measures:
mRNAs were quantified by real-time RT-PCR. Promoter reporter constructs activities were quantified by the measurement of their luciferase activity.
Results:
BMP15 and BMP4 enhanced AMHR2 and AMH expression in human GCs and in oGCs, whereas GDF9 had no effect. In oGCs, GDF9 increased BMP15 effect on AMH expression. Consistent with these results, BMP15 and BMP4, but not GDF9, enhanced AMHR2 promoter activity in oGCs, whereas GDF9 increased BMP15 effect on AMH promoter activity. Moreover, oGCs from both BMP15 mutant ewes had reduced AMHR2 mRNA levels but unchanged AMH expression compared with wild-type ewes.
Conclusions:
Altogether, these results suggest that the mechanisms of action of BMP15 on AMHR2 and AMH expression are different, and that by stimulating AMHR2 and AMH expression in GCs BMP15 enhances AMH inhibitory actions in GCs.
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