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Characterization of GATA/GACA-related sequences on proximal chromosome 17 of the mouse
E J Durbin1, R P Erickson, A Craig
1Department of Human Genetics, University of Michigan Medical School, Ann Arbor 48109.
Abstract:
Autosomal loci have long been thought to have a role in sex determination of mice. We studied the localization of GATA/GACA repeats on chromosome 17 in regard to the possibility of their involvement in sex determination. We performed in situ hybridizations on chromosome 17s carrying the Hairpain tail (Thp) deletion of the T locus since this deletion has been associated with sex reversal and hermaphroditism. We did not detect a significant decrease in the amount of hybridization of GATA/GACA repeats to the Thp deletion. In addition, three Bkm-positive cosmids from proximal chromosome 17 did not contain sequences deleted in Thp or TOrl and a fetal testes cDNA probe did not hybridize to the cosmid sequences. Although we confirmed the localization of Bkm-related sequences on chromosome 17, we were not able to relate GATA/GACA sequences on chromosome 17 to sex determination in mice.