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The Olfactory System as a Model to Study Axonal Growth Patterns and Morphology In Vivo
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RNA-seq analysis of developing olfactory bulb projection neurons.

Yuka Imamura Kawasawa1, Anna C Salzberg2, Mingfeng Li3

  • 1Department of Pharmacology, Pennsylvania State University College of Medicine, 500 University Dr., Hershey, PA 17033, USA; Department of Biochemistry and Molecular Biology, Pennsylvania State University College of Medicine, 500 University Dr., Hershey, PA 17033, USA; Institute for Personalized Medicine, Pennsylvania State University College of Medicine, 500 University Dr., Hershey, PA 17033, USA.

Molecular and Cellular Neurosciences
|April 14, 2016
PubMed
Summary

Researchers identified key genes and splicing events in developing olfactory bulb (OB) projection neurons, revealing molecular insights into mitral and tufted cell development and their relation to cortical neurons.

Keywords:
Alternative splicingCell-type specific transcriptomeMitral cellNeuronal developmentOlfactory bulbPyramidal neuronRNA-seq

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Area of Science:

  • Neuroscience
  • Developmental Biology
  • Genomics

Background:

  • Olfactory bulb (OB) projection neurons, mitral and tufted cells, transmit olfactory information.
  • Their developmental molecular networks are largely unknown.
  • Mitral/tufted cells share similarities with cortical projection neurons but have unique features.

Purpose of the Study:

  • To investigate the developmental gene expression patterns in OB projection neurons.
  • To identify molecular mechanisms regulating mitral and tufted cell development.

Main Methods:

  • Purified OB projection neurons were analyzed using next-generation RNA sequencing (RNA-seq) at various developmental stages.
  • Gene expression profiles were compared between olfactory projection neurons and cortical projection neuron subtypes.

Main Results:

  • 1202 protein-coding genes showed temporal differential regulation in developing projection neurons; 388 were specific to these neurons.
  • Developing olfactory projection neurons share molecular signatures with subcerebral neurons.
  • 3422 splicing isoform ratio changes were identified, including novel isoforms.

Conclusions:

  • The study provides a comprehensive resource for understanding OB projection neuron development.
  • Identified genes and splicing events offer insights into mitral and tufted cell maturation.
  • Molecular similarities suggest conserved developmental pathways between olfactory and subcerebral neurons.