Streptosporangium becharense sp. nov., an actinobacterium isolated from desert soil

The taxonomic position of a novel actinobacterium, strain SG1^T, isolated from a Saharan soil sample collected from Béni-Abbès, Béchar (south-west Algeria), was established by using a polyphasic approach. The micro-organism had morphological and chemical features that were consistent with its classification in the genus Streptosporangium. The cell-wall peptidoglycan contained meso-diaminopimelic acid. The whole-cell sugars contained ribose and glucose, but not madurose. The predominant menaquinones were MK-9(H2) and MK-9(H4). The polar lipid profile contained diphosphatidylglycerol, phosphatidylmethylethanolamine, phosphatidylethanolamine, phosphatidylhydroxymethylethanolamine, phosphatidylhydroxyethanolamine, phosphatidylglycerol, phosphatidylinositol and phosphatidylinositol mannosides. The predominant cellular fatty acids were C17 : 1ω8c, iso-C16 : 0, 10-methyl C17 : 0, C18 : 1ω9c and C17 : 0. 16S rRNA gene sequence similarity analysis supported the classification of the isolate in the genus Streptosporangium and indicated that it was related most closely to 'Streptosporangium subfuscum' DSM 46724 (99.7 % similarity), Streptosporangium pseudovulgare DSM 43181^T (98.7 %), Streptosporangium fragile DSM 43847^T (98.6 %) and Streptosporangium sandarakinum DSM 45763^T (98.5 %). Phylogenetic analyses based on 16S rRNA gene sequences showed that strain SG1^T formed a cluster with its closest relative 'S. subfuscum' DSM 46724. However, DNA-DNA relatedness as well as physiological and chemotaxonomical analyses showed that strain SG1^T could be differentiated from its closest phylogenetic relatives. Therefore, it is proposed that strain SG1^T should be classified as representing a novel species in the genus Streptosporangium, for which the name Streptosporangiumbecharense sp. nov. is proposed. The type strain is SG1^T (=DSM 46887^T=CECT 8961^T).