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Calcium-dependent binding between calmodulin and lysozyme
J F Fierro1, M A Pajares, C Hardisson
1Departamento de Biologia Funcional, Facultad de Medicina, Universidad de Oviedo, Spain.
FEBS Letters
|April 10, 1989
Abstract:
Calmodulin, an acidic protein that binds calcium with high affinity, has multiple roles in the activation of many enzymes involved in cellular regulation of eukaryotes. In this study we show that calmodulin binding to hen egg-white lysozyme, in a Ca2+-dependent way, was observed using electroblots incubated with biotinylated calmodulin and detected with avidin-alkaline phosphatase or for affinity chromatography on a gel calmodulin column. Antimicrobial activity of lysozyme was not modified in the presence of Ca2+-calmodulin.