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Published on: July 16, 2019
Analysis on the transcriptome information of two different wheat mutants and identification of salt-induced
Xiaoli Ma1, Peihan Gu1, Wenji Liang2
1College of Life Science, Hebei Normal University, Shijiazhuang 050024 Hebei, PR China.
Abstract:
This study established a wheat transcriptome library using RH8706-49 and RH8706-34. Salt-induced differential genes were screened by Illumina RNA sequencing (RNA-Seq). Five differential genes were chosen to study the functions by combining transcript sequencing result and gene chip. The expression changes of these five differential genes were analyzed using real-time quantitative PCR (qRT-PCR) technique to determine the reliability and accuracy of transcriptome sequencing and transplanted into Arabidopsis thaliana to obtain transgenic homozygote plants for the salt tolerance test. The salt tolerance test results show that the transgenic plants grew far better than the wild-type plant.
