Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Production of Pharmaceuticals01:30

Production of Pharmaceuticals

22
Industrial insulin production uses genetically engineered E. coli expressing a proinsulin gene controlled by a tryptophan promoter and containing a methionine linker for later cleavage. The cells also carry ampicillin resistance for selective growth. Seed cultures are stored at −80 °C and production begins by thawing a small amount to inoculate starter cultures, which are progressively scaled to a 50,000-L bioreactor. In the bioreactor, E. coli grow in nutrient-rich media under...
22
Directly Acting Muscle Relaxants: Dantrolene and Botulinum Toxin01:26

Directly Acting Muscle Relaxants: Dantrolene and Botulinum Toxin

1.4K
Directly acting muscle relaxants like dantrolene and botulinum toxin (BoNT) have distinct mechanisms and applications. Dantrolene, a hydantoin derivative, acts on the ryanodine receptor (RYR1) in skeletal muscle cells. RYR1 are calcium channels present at the sarcoplasmic reticulum membrane. In response to excitation, they release calcium ions from the sarcoplasmic reticulum to the cytosol. Calcium promotes actin-myosin-mediated contraction of muscles.
The binding of dantrolene to the RYR1...
1.4K
Production of Biopesticides01:18

Production of Biopesticides

14
Biopesticides offer a sustainable alternative to chemical pesticides, utilizing microbial agents to control agricultural pests. Bacillus thuringiensis (Bt) is a widely employed bacterium known for its potent insecticidal activity. Bt biopesticides are favored for their specificity to insect pests, minimal environmental impact, and natural degradability.Mechanism of Bt Toxin Action Bt produces insecticidal crystal (Cry) proteins during its sporulation phase. These proteins form parasporal...
14
Recombinant DNA01:09

Recombinant DNA

104.6K
Overview
104.6K
Bacterial Toxins01:12

Bacterial Toxins

23
Bacterial toxins are sophisticated virulence factors that enable pathogenic bacteria to interact with, invade, and damage host tissues. These toxins fall broadly into two types: protein exotoxins, which are secreted into the environment and target specific host receptors, and lipopolysaccharide endotoxins, which are structural components of the bacterial outer membrane released primarily during bacterial lysis or membrane shedding. Exotoxins generally act more selectively, binding to cell...
23
Vaccine Production01:23

Vaccine Production

30
Vaccine production involves a sequence of upstream and downstream processes to generate a safe and effective immunological product. It begins with cultivating microorganisms, such as viruses or bacteria, to obtain antigenic material. For viral vaccines, mammalian host cells are grown in bioreactors and subsequently infected with the target virus. The virus replicates within the host cells, which are lysed to release viral particles. This lysate is then clarified through filtration or...
30

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Antibiotic susceptibility of lactic acid bacteria isolated from artisanal coalho cheese in the Agreste region of Pernambuco, Brazil.

Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology]·2026
Same author

The problem of segmenting global glomerulosclerosis in gigapixel histopathological images: the borderless glomeruli.

BMC nephrology·2025
Same author

Komagataella pastoris KM71H Attenuates Cognitive Deficits and Depressive-Like Behavior by the TLR4/NF-κB Signaling Pathway in an Aβ<sub>1-40</sub>-Induced AD-Like Mouse Model.

Molecular neurobiology·2025
Same author

Abnormal hyperactivity of specific striatal ensembles encodes distinct dyskinetic behaviors revealed by high-resolution clustering.

Cell reports·2025
Same author

Differential Effects of Levodopa and Stimulation on Post-Surgery Freezing of Gait in Subthalamic Nucleus Deep Brain Stimulation Parkinson's Disease Patients: A Clinical and Kinematic Analysis.

Movement disorders clinical practice·2025
Same author

Phenotypical and molecular characterization of Rhodococcus equi isolated from foals in the Agreste region of Pernambuco - Brazil.

Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology]·2025

Related Experiment Video

Updated: Mar 22, 2026

A High-throughput-compatible FRET-based Platform for Identification and Characterization of Botulinum Neurotoxin Light Chain Modulators
10:30

A High-throughput-compatible FRET-based Platform for Identification and Characterization of Botulinum Neurotoxin Light Chain Modulators

Published on: December 27, 2013

5.8K

Recombinant Botulinum Toxoids: A Practical Guide for Production.

Gustavo Marçal S G Moreira1, Clóvis Moreira1, Carlos Eduardo P da Cunha1

  • 1Laboratório de Imunologia Aplicada, Núcleo de Biotecnologia, Centro de Desenvolvimento Tecnológico, Universidade Federal de Pelotas, Pelotas, Rio Grande do Sul, CEP 96160-000, Brazil.

Methods in Molecular Biology (Clifton, N.J.)
|April 15, 2016
PubMed
Summary

Developing recombinant vaccines using Escherichia coli offers a safer and faster alternative to traditional Clostridium botulinum vaccines for animals. This method simplifies production and reduces biosafety risks associated with toxoids and bacterins.

Keywords:
Botulinum neurotoxinsBotulismClostridium botulinumEscherichia coliRecombinant toxoidRecombinant vaccine

More Related Videos

Isolation and Quantification of Botulinum Neurotoxin From Complex Matrices Using the BoTest Matrix Assays
12:25

Isolation and Quantification of Botulinum Neurotoxin From Complex Matrices Using the BoTest Matrix Assays

Published on: March 3, 2014

16.6K
A High Content Imaging Assay for Identification of Botulinum Neurotoxin Inhibitors
14:10

A High Content Imaging Assay for Identification of Botulinum Neurotoxin Inhibitors

Published on: November 14, 2014

9.0K

Related Experiment Videos

Last Updated: Mar 22, 2026

A High-throughput-compatible FRET-based Platform for Identification and Characterization of Botulinum Neurotoxin Light Chain Modulators
10:30

A High-throughput-compatible FRET-based Platform for Identification and Characterization of Botulinum Neurotoxin Light Chain Modulators

Published on: December 27, 2013

5.8K
Isolation and Quantification of Botulinum Neurotoxin From Complex Matrices Using the BoTest Matrix Assays
12:25

Isolation and Quantification of Botulinum Neurotoxin From Complex Matrices Using the BoTest Matrix Assays

Published on: March 3, 2014

16.6K
A High Content Imaging Assay for Identification of Botulinum Neurotoxin Inhibitors
14:10

A High Content Imaging Assay for Identification of Botulinum Neurotoxin Inhibitors

Published on: November 14, 2014

9.0K

Area of Science:

  • Veterinary immunology
  • Microbial biotechnology
  • Toxicology

Background:

  • Clostridium botulinum produces potent neurotoxins (BoNTs), with serotypes C and D being significant in veterinary medicine.
  • Current vaccines against botulism in animals use inactivated toxins or cells, facing challenges like lengthy production, variable yields, and biosafety concerns.

Purpose of the Study:

  • To describe a method for producing recombinant BoNT vaccines for veterinary use.
  • To present Escherichia coli as a viable expression system for efficient and safe vaccine antigen production.

Main Methods:

  • Gene design for recombinant BoNT antigens.
  • Production of nontoxic recombinant antigens using Escherichia coli expression systems.
  • Vaccine formulation and evaluation for veterinary applications.

Main Results:

  • Escherichia coli enables rapid production of large quantities of recombinant BoNT antigens.
  • Recombinant vaccine production simplifies processes and minimizes biosafety risks compared to traditional methods.
  • The described method covers the entire process from gene design to vaccine evaluation.

Conclusions:

  • Recombinant vaccines produced in E. coli are a promising alternative for controlling animal botulism.
  • This approach addresses the limitations of current commercial vaccines, offering improved safety and efficiency.
  • The developed method provides a streamlined pathway for veterinary vaccine production.