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Quantification of Proteins Using Peptide Immunoaffinity Enrichment Coupled with Mass Spectrometry
Published on: July 31, 2011
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Proximity assays for sensitive quantification of proteins.
Christina Greenwood1, David Ruff2, Sara Kirvell1
1Postgraduate Medical Institute, Faculty of Medical Science, Anglia Ruskin University, Chelmsford, Essex CM1 1SQ, UK.
Biomolecular Detection and Quantification
|April 15, 2016
Summary
Proximity assays use DNA-tagged molecules to detect proteins and their interactions. These sensitive immunohistochemical tools offer accurate assessments for research and diagnostics.
Area of Science:
- Biochemistry
- Molecular Biology
- Immunohistochemistry
Background:
- Proximity assays are immunohistochemical tools employing DNA-tagged aptamers or antibodies.
- These tools bind to proteins or protein complexes in close proximity.
Purpose of the Study:
- To describe the principles and applications of proximity assays.
- To highlight their potential in sensitive and specific protein detection.
Main Methods:
- Utilizing DNA-tagged aptamers or antibodies for proximity binding.
- Signal generation through amplification (PCR or isothermal) and probe hybridization.
- Assays can be performed in homogeneous, solid-phase, or in situ formats.
Main Results:
- Enables sensitive and robust detection of proteins, protein modifications, and protein-protein interactions.
- In situ assays provide high spatial accuracy for visualizing single protein molecules or complexes.
Conclusions:
- Proximity assays offer sensitive, specific, and accurate protein expression assessments.
- Their properties make them valuable for research, diagnostics, and pharmacology.
