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Related Experiment Video

Updated: Mar 22, 2026

Multi-timescale Microscopy Methods for the Characterization of Fluorescently-labeled Microbubbles for Ultrasound-Triggered Drug Release

Multi-timescale Microscopy Methods for the Characterization of Fluorescently-labeled Microbubbles for Ultrasound-Triggered Drug Release

Published on: June 12, 2021

Shell thickness determination of polymer-shelled microbubbles using transmission electron microscopy.

Johan Härmark¹, Hans Hebert¹, Philip J B Koeck¹

¹School of Technology and Health, KTH Royal Institute of Technology and Department of Biosciences and Nutrition, Karolinska Institutet, Stockholm, Sweden.

Micron (Oxford, England : 1993)

|April 15, 2016

Summary

Researchers developed a new dual-mode contrast agent (CA) using microbubbles (MBs) decorated with nanoparticles for enhanced ultrasound and MRI imaging. A mathematical model accurately determined the average shell thickness of these novel MBs, crucial for acoustic properties.

Keywords:

Contrast agent Microbubbles Shell thickness Transmission electron microscopy

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Last Updated: Mar 22, 2026

Multi-timescale Microscopy Methods for the Characterization of Fluorescently-labeled Microbubbles for Ultrasound-Triggered Drug Release

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In situ TEM of Biological Assemblies in Liquid

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Published on: December 30, 2013

Area of Science:

Biomedical Engineering
Materials Science
Medical Imaging

Background:

Microbubbles (MBs) are used as ultrasound contrast agents (CAs) to improve image quality.
A novel air-filled MB CA stabilized with polyvinyl alcohol (PVA) has been developed.
These MBs are decorated with superparamagnetic iron oxide nanoparticles (SPIONs) for dual ultrasound and MRI imaging capabilities.

Purpose of the Study:

To introduce a mathematical model for determining the shell thickness of SPIONs-decorated MBs (Type A and Type B).
To investigate the impact of shell thickness on the acoustical properties of MBs.
To validate the mathematical model using simulated data.

Main Methods:

Preparation of thin sections of plastic-embedded MBs.
Imaging of MB sections using transmission electron microscopy (TEM).
Development and application of a mathematical model to calculate average shell thickness from corrected TEM data.

Main Results:

The mathematical model accurately determined the average shell thickness of SPIONs-decorated MBs.
The average shell thickness for Type A MBs was 651nm.
The average shell thickness for Type B MBs was 637nm.

Conclusions:

The developed mathematical model provides an accurate method for determining MB shell thickness.
Accurate shell thickness measurement is critical for understanding and optimizing the acoustic properties of dual-mode CAs.
This work contributes to the advancement of novel contrast agents for multimodal medical imaging.