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Important roles played by TGF-β member of Bmdpp and Bmdaw in BmNPV infection

Xiaolong Hu1, Yue Jiang2, Yongchang Gong2

  • 1School of Biology & Basic Medical Science, Soochow University, Suzhou 215123, China; National Engineering Laboratory for Modern Silk, Soochow University, Suzhou 215123, China.

Molecular Immunology
|April 15, 2016
PubMed

Insights

Transforming growth factor-beta (TGF-β) members Decapentaplegic (Bmdpp) and Dawdle (Bmdaw) regulate Bombyx mori nucleohedrovirus (BmNPV) replication. Overexpression inhibits viral multiplication, while gene silencing enhances it in silkworms.

Area of Science:

  • Entomology
  • Molecular Biology
  • Virology

Background:

  • The role of Transforming Growth Factor-beta (TGF-β) superfamily members in inhibiting Bombyx mori nucleohedrovirus (BmNPV) multiplication remains unclear.
  • Silkworms (Bombyx mori) are economically important insects susceptible to viral infections.

Purpose of the Study:

  • To investigate the function of TGF-β superfamily members, specifically Decapentaplegic (Bmdpp) and Dawdle (Bmdaw), in the silkworm's response to BmNPV infection.
  • To determine how Bmdpp and Bmdaw regulate viral RNA transcription, protein expression, and multiplication.

Main Methods:

  • Quantitative analysis of BmNPV RNA transcription and protein expression.
  • Subcellular localization and tissue expression pattern analysis of Bmdpp and Bmdaw.
  • Overexpression and RNA interference (RNAi) experiments in silkworm ovary-derived cells (BmN cells) and silkworms.
  • Analysis of antiviral immune pathway regulation.

Main Results:

  • Bmdpp and Bmdaw regulate BmNPV RNA transcription and protein expression in B. mori.
  • Bmdpp and Bmdaw are primarily localized in the cytomembrane and highly expressed in hemocytes.
  • Overexpression of Bmdpp and Bmdaw inhibited BmNPV multiplication, while RNAi enhanced it.
  • Antiviral immune pathways were not significantly affected by these TGF-β members.

Conclusions:

  • Bmdpp and Bmdaw play a crucial role in the silkworm's innate immune response against BmNPV infection.
  • These findings provide insights into the antiviral mechanisms involving TGF-β superfamily members in insects.
Keywords:
BmNPVBombyx moriTGF-&betadawdledecapentaplegic

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