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Related Experiment Video

Updated: Mar 22, 2026

Whisker-signaled Eyeblink Classical Conditioning in Head-fixed Mice
10:14

Whisker-signaled Eyeblink Classical Conditioning in Head-fixed Mice

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Whisker-signaled Eyeblink Classical Conditioning in Head-fixed Mice.

Carmen Lin1, John Disterhoft1, Craig Weiss2

  • 1Department of Physiology, Northwestern University.

Journal of Visualized Experiments : Jove
|April 15, 2016
PubMed
Summary

This study presents a head-fixed apparatus for training mice in eyeblink conditioning. This method allows for cellular-level investigation of learning and memory mechanisms.

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Area of Science:

  • Neuroscience
  • Behavioral Neuroscience
  • Learning and Memory Research

Background:

  • Eyeblink conditioning is crucial for studying learning and memory neural mechanisms.
  • Mice typically undergo free-moving training, limiting cellular-level investigation.
  • A stable cranial platform is needed for advanced cellular studies.

Purpose of the Study:

  • To develop a head-fixed training apparatus for mice in eyeblink conditioning.
  • To enable cellular-level investigation of learning and memory in mice.
  • To facilitate the use of advanced scientific techniques in memory research.

Main Methods:

  • Mice were trained using a head-fixed apparatus allowing voluntary running.
  • Whisker stimulation served as the conditioned stimulus (CS).
  • Periorbital electrical shock was used as the unconditioned stimulus (US), with electromyographic (EMG) activity recorded.

Main Results:

  • The head-fixed apparatus enabled reliable eyeblink conditioning in mice.
  • The system facilitated precise delivery of stimuli and recording of responses.
  • This setup supports detailed cellular and neural circuit analysis.

Conclusions:

  • The developed head-fixed apparatus is effective for mouse eyeblink conditioning.
  • This method advances the study of learning and memory at the cellular level.
  • It provides a valuable tool for neuroscience research.