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Related Experiment Video

Updated: Mar 22, 2026

Imaging Subcellular Structures in the Living Zebrafish Embryo

Imaging Subcellular Structures in the Living Zebrafish Embryo

Published on: April 2, 2016

Imaging Subcellular Structures in the Living Zebrafish Embryo.

Peter Engerer¹, Gabriela Plucinska², Rachel Thong³

¹Institute of Neuronal Cell Biology, Technische Universität München; Peter.Engerer@tum.de.

Journal of Visualized Experiments : Jove

|April 15, 2016

Summary

Zebrafish embryos offer a non-invasive way to study dynamic cellular and subcellular processes using in vivo imaging. This study details methods for fluorescently labeling organelles, enabling advanced cell biology research in live zebrafish.

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Published on: July 25, 2010

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Last Updated: Mar 22, 2026

Imaging Subcellular Structures in the Living Zebrafish Embryo

Imaging Subcellular Structures in the Living Zebrafish Embryo

Published on: April 2, 2016

Live Imaging of the Zebrafish Embryonic Brain by Confocal Microscopy

Live Imaging of the Zebrafish Embryonic Brain by Confocal Microscopy

Published on: April 1, 2009

Labeling and Imaging Cells in the Zebrafish Hindbrain

Labeling and Imaging Cells in the Zebrafish Hindbrain

Published on: July 25, 2010

Area of Science:

Cell Biology
Developmental Biology
Microscopy

Background:

In vivo imaging allows observation of cellular dynamics in natural environments.
Mammalian studies often require invasive surgical procedures for imaging.
Zebrafish embryos provide optical accessibility, circumventing the need for surgery.

Purpose of the Study:

To describe methods for generating genetic constructs for fluorescently labeling organelles in zebrafish.
To demonstrate the utility of zebrafish as a model for in vivo cell biology.
To provide guidelines for imaging subcellular dynamics in zebrafish.

Main Methods:

Utilizing the bipartite Gal4-UAS system for cell-type-specific gene expression.
Generating protocols for transiently expressing and stable transgenic zebrafish lines.
Developing methods for fluorescently labeling mitochondria and centrosomes.

Main Results:

Successful generation of genetic constructs for organelle labeling.
Demonstration of imaging subcellular events like mitochondrial trafficking and centrosome dynamics.
Establishment of zebrafish as a versatile model for in vivo subcellular research.

Conclusions:

Zebrafish are a powerful model for non-invasive in vivo imaging of cellular and subcellular dynamics.
Fluorescent labeling techniques and light microscopy enable detailed study of organelle behavior.
This approach advances the field of cell biology by providing new avenues for research.