Related Experiment Videos
Effect of heparin administration on flunitrazepam protein binding
R Calvo1, L Aguilera, C Aguirre
1Department of Pharmacology, University of Basque Country, Leioa, Vizcaya, Spain.
International Journal of Clinical Pharmacology Research
|January 1, 1989
Summary
Free fatty acids significantly decrease flunitrazepam binding to plasma proteins. This finding is crucial for understanding drug interactions and flunitrazepam
Area of Science:
- Pharmacology
- Biochemistry
- Clinical Chemistry
Background:
- Non-esterified fatty acids displace diazepam from plasma binding sites.
- Lorazepam binding is unaffected by free fatty acids.
- Flunitrazepam shows significant plasma protein binding.
Purpose of the Study:
- To investigate the effect of free fatty acids on flunitrazepam binding to human plasma proteins.
Main Methods:
- In vitro incubation of plasma with sodium oleate.
- In vivo administration of sodium heparin to increase free fatty acid levels.
- Measurement of flunitrazepam free fraction in plasma.
Main Results:
- Sodium oleate decreased flunitrazepam binding, increasing its free fraction.
- Heparin administration increased free fatty acids and flunitrazepam free fraction.
- A direct relationship was observed between free fatty acid concentration and flunitrazepam's unbound fraction.
Conclusions:
- Increased free fatty acid concentrations reduce the plasma protein binding of flunitrazepam.
- These findings highlight a potential drug interaction mechanism involving free fatty acids and flunitrazepam.