Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Soluble extracts from larval Ostertagia ostertagi modulating immune function.

D A Cross, P H Klesius

    International Journal for Parasitology
    |February 1, 1989
    PubMed
    Summary

    A specific Ostertagia ostertagi antigen suppressed antibody production and B cell activity in mice. This immune-suppressive antigen also reduced lymphocyte responses to Concanavalin A stimulation.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Mechanisms of laser-induced damage in absorbing glasses with nanosecond pulses.

    Optics express·2019
    Same author

    Mechanisms of surface contamination in fused silica by means of laser-induced electrostatic effects.

    Optics letters·2017
    Same author

    Identification of gyrB and rpoB gene mutations and differentially expressed proteins between a novobiocin-resistant Aeromonas hydrophila catfish vaccine strain and its virulent parent strain.

    Veterinary microbiology·2013
    Same author

    Chemotactic activity of channel catfish, Ictalurus punctatus (Rafinesque), recombinant cyclophilin A.

    Journal of fish diseases·2013
    Same author

    Identification and virulence of Chryseobacterium indologenes isolated from diseased yellow perch (Perca flavescens).

    Journal of applied microbiology·2012
    Same author

    Reproducible challenge model to investigate the virulence of Flavobacterium columnare genomovars in rainbow trout Oncorhynchus mykiss.

    Diseases of aquatic organisms·2012

    Area of Science:

    • Immunology
    • Parasitology
    • Veterinary Science

    Background:

    • Ostertagia ostertagi is a significant gastrointestinal nematode parasite affecting livestock.
    • Understanding host immune responses to parasitic antigens is crucial for developing effective control strategies.
    • Immune modulation by parasite antigens can impact host susceptibility and vaccine efficacy.

    Purpose of the Study:

    • To investigate the immunomodulatory effects of Ostertagia ostertagi larval antigens on antibody production and lymphocyte function in BALB/c mice.
    • To identify specific antigens responsible for immune suppression.
    • To evaluate the impact of these antigens on B cell activity and T cell proliferation.

    Main Methods:

    • BALB/c mice were immunized with O. ostertagi antigens and T-dependent antigens (KLH or SR).

    Related Experiment Videos

  • Antibody production was assessed using enzyme-linked immunosorbent assay (ELISA).
  • B cell activity was measured by modified Jerne plaque assay, and lymphocyte blastogenesis was evaluated using Concanavalin A stimulation in vitro.

    • Main Results:

    • A semi-purified larval antigen significantly decreased anti-keyhole limpet hemocyanin (KLH) serum antibody levels.
    • The same antigen reduced the number of anti-sheep erythrocytes (SR) IgM-secreting B cells.
    • This antigen suppressed lymphocyte blastogenesis in cultured splenic lymphocytes stimulated with Concanavalin A.

    Conclusions:

    • Certain Ostertagia ostertagi larval antigens possess immunomodulatory properties that can suppress host antibody production and B cell function.
    • These antigens may interfere with T cell activation, as indicated by reduced lymphocyte blastogenesis.
    • Further research into these immunosuppressive antigens could inform vaccine development and parasite control strategies.