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Asymmetric Lipid Bilayer

Asymmetric Lipid Bilayer

Biological membranes show uneven distribution of different types of lipids in the inner and outer layers, resulting in transverse asymmetric membranes. The treatment of the erythrocyte membrane with the enzyme phospholipase confirmed the asymmetric nature of the lipid bilayer. The enzyme hydrolyzes lipids into fatty acids and hydrophilic groups. The phospholipase acts only on the outer layer of the membrane, while the inner layer remains intact. The phospholipase treatment resulted in 80%...

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Updated: Mar 22, 2026

Temperature-Controlled Assembly and Characterization of a Droplet Interface Bilayer

Temperature-Controlled Assembly and Characterization of a Droplet Interface Bilayer

Published on: April 19, 2021

Arrayed water-in-oil droplet bilayers for membrane transport analysis.

R Watanabe¹, N Soga², M Hara²

¹Department of Applied Chemistry, The University of Tokyo, PRESTO, Japan Science and Technology Agency, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-8656, Japan. wrikiya@nojilab.t.u-tokyo.ac.jp.

|April 16, 2016

Summary

This study introduces a micro-device for creating over 500 femtoliter droplet interface bilayers, enabling controlled droplet volumes for enhanced membrane transport analysis and drug screening.

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Area of Science:

Biophysics
Materials Science
Analytical Chemistry

Background:

Water-in-oil droplet bilayers are valuable for membrane transport studies.
Current methods struggle with controlling droplet size for high-throughput analysis.

Purpose of the Study:

To develop a novel micro-device for high-throughput droplet interface bilayer formation.
To enable precise control over femtoliter droplet volumes for enhanced analysis.

Main Methods:

Fabrication of a micro-device with immobilized femtoliter-sized droplet arrays.
Utilizing hydrophobic/hydrophilic patterns to control droplet volumes (3.5-350 fL).
Formation of over 500 droplet interface bilayers simultaneously.

Main Results:

Achieved controllable droplet volumes in the femtoliter range.
Enabled high-throughput formation of droplet interface bilayers (>500).
Demonstrated potential for analyzing membrane permeability and transport proteins.

Conclusions:

The novel micro-device significantly improves droplet interface bilayer technology.
This platform enhances membrane transport analysis and offers new possibilities for drug screening and analytical applications.