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Related Experiment Video

Updated: Mar 22, 2026

Assessing Primary Neurogenesis in Xenopus Embryos Using Immunostaining

Assessing Primary Neurogenesis in Xenopus Embryos Using Immunostaining

Published on: April 12, 2016

TOX3 regulates neural progenitor identity.

Sanjeeb Kumar Sahu¹, Alina Fritz², Neha Tiwari³

¹Institute of Molecular Biology, (IMB), Mainz, Germany.

Biochimica Et Biophysica Acta

|April 16, 2016

Summary

The transcription factor TOX3 is crucial for developing mouse brains, supporting neural stem cell function and migration. This finding sheds light on TOX3's role in nervous system development.

Keywords:

Neuronal differentiation Neuronal progenitors Notch Proliferation Transcription

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Area of Science:

Neuroscience
Developmental Biology
Genetics

Background:

The function of the transcription factor TOX3 is largely unknown, despite its association with restless legs syndrome and breast cancer risk.
TOX3's specific role in physiological processes requires further investigation.

Purpose of the Study:

To investigate the physiological role of the transcription factor TOX3 in the developing nervous system.

Main Methods:

Analyzed Tox3 expression in developing mouse brains using immunohistochemistry.
Performed chromatin immunoprecipitation assays to assess Tox3 binding to the Nestin promoter.
Utilized in utero electroporation to knock down Tox3 expression in embryonic mouse brains.

Main Results:

Tox3 is predominantly expressed in the developing mouse brain, co-localizing with neural stem and progenitor markers.
Tox3 binds the Nestin promoter and regulates its activity, influenced by Notch signaling.
Tox3 knockdown impairs Nestin expression, neural progenitor proliferation, and migration.

Conclusions:

Tox3 plays a significant role in nervous system development by regulating neural stem and progenitor cells.
These findings provide novel insights into the function of TOX3 in neurodevelopment.