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Related Experiment Video

Updated: Mar 22, 2026

Chemiluminescence-based Assays for Detection of Nitric Oxide and its Derivatives from Autoxidation and Nitrosated Compounds

Chemiluminescence-based Assays for Detection of Nitric Oxide and its Derivatives from Autoxidation and Nitrosated Compounds

Published on: February 16, 2022

Acrylic microspheres-based optosensor for visual detection of nitrite.

Nur Syarmim Mohamed Noor¹, Ling Ling Tan², Lee Yook Heng²

¹Faculty of Industrial Sciences & Technology, Universiti Malaysia Pahang (UMP), Lebuhraya Tun Razak, 26300 Gambang, Kuantan, Pahang Darul Makmur, Malaysia.

|April 16, 2016

Summary

This summary is machine-generated.

A novel optosensor utilizes Safranine O immobilized on poly(n-butyl acrylate) microspheres for visual nitrite detection. This method offers reproducible and sensitive quantification of nitrite ions in food samples.

Keywords:

Nitrite ion Optosensor Poly(n-butyl acrylate) microspheres Reflectance spectrophotometer Safranine O

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Area of Science:

Analytical Chemistry
Materials Science
Sensor Technology

Background:

Nitrite ion (NO2(-)) determination is crucial in food safety.
Existing methods for nitrite detection can be complex or require specialized equipment.
Development of simple, visual, and sensitive optosensors is desirable.

Purpose of the Study:

To develop a novel optosensor for the visual quantitation of nitrite ions.
To immobilize Safranine O (SO) reagent onto a poly(n-butyl acrylate) (poly(nBA)) microsphere matrix.
To evaluate and optimize the performance of the fabricated optosensor.

Main Methods:

Fabrication of poly(nBA) microspheres via microemulsion UV lithography.
Physical immobilization of Safranine O reagent onto the microspheres.
Characterization using scanning electron microscopy (SEM).
Optimization and evaluation using a fiber optic reflectance spectrophotometer.

Main Results:

Poly(nBA) microspheres exhibited smooth spherical morphology with particle sizes ranging from 0.6 to 1.8 μm.
Uniform microsphere size ensured homogeneous SO immobilization, leading to high reproducibility (<5% RSD).
The optosensor demonstrated a linear detection range for nitrite ions from 10 to 100 ppm.
The microsphere matrix facilitated efficient ion transport for reflectometric determination.

Conclusions:

A new optosensor based on SO-immobilized poly(nBA) microspheres was successfully developed.
The sensor provides a reproducible and sensitive method for visual nitrite quantitation.
This approach is suitable for reflectometric nitrite determination in food samples.