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Identification of Rare Bacterial Pathogens by 16S rRNA Gene Sequencing and MALDI-TOF MS
06:34

Identification of Rare Bacterial Pathogens by 16S rRNA Gene Sequencing and MALDI-TOF MS

Published on: July 11, 2016

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High-quality draft genome sequence and description of Haemophilus massiliensis sp. nov

Cheikh Ibrahima Lo1, Senthil Alias Sankar2, Bécaye Fall3

  • 1Aix-Marseille Université, URMITE, UM63, CNRS 7278, IRD 198, Inserm U1095, Faculté de médecine, Marseille, France ; Campus international UCAD-IRD, Dakar, Senegal.

Standards in Genomic Sciences
|April 16, 2016
PubMed

Insights

A novel bacterium, Haemophilus massiliensis, was identified from pelvic peritonitis fluid. This Gram-negative bacterium represents a new species within the Pasteurellaceae family, expanding our understanding of microbial diversity.

Area of Science:

  • Microbiology
  • Bacteriology
  • Genomics

Background:

  • Pelvic peritonitis can be caused by various bacterial pathogens.
  • Accurate identification of causative agents is crucial for effective treatment.

Observation:

  • A novel bacterial strain, FF7(T), was isolated from a patient with pelvic peritonitis.
  • Strain FF7(T) showed low 16S rRNA sequence identity (94.8%) with Haemophilus parasuis and poor MALDI-TOF MS identification scores.

Findings:

  • Polyphasic analysis characterized strain FF7(T) as a Gram-negative, facultatively anaerobic rod.
  • Genomic analysis revealed a 2.44 Mbp genome with 2,319 protein-coding genes and 67 RNA genes.
  • Based on phenotypic and genomic data, strain FF7(T) is proposed as the type strain of a new species, Haemophilus massiliensis.

Implications:

  • The discovery of Haemophilus massiliensis expands the known diversity within the Pasteurellaceae family.
  • This finding may contribute to a better understanding of the microbial etiology of peritonitis.
  • Further research is needed to determine the clinical significance and pathogenic potential of this new species.
Keywords:
CulturomicsGenomeHaemophilus massiliensisTaxono-genomics

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