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Updated: Mar 22, 2026

Integration Free Derivation of Human Induced Pluripotent Stem Cells Using Laminin 521 Matrix
Published on: July 7, 2017
Engineering human cells and tissues through pluripotent stem cells
Jeffrey R Jones1, Su-Chun Zhang2
1Waisman Center, University of Wisconsin, Madison, WI 53705, United States.
Abstract:
The utility of human pluripotent stem cells (hPSCs) depends on their ability to produce functional cells and tissues of the body. Two strategies have been developed: directed differentiation of enriched populations of cells that match a regional and functional profile and spontaneous generation of three-dimensional organoids that resemble tissues in the body. Genomic editing of hPSCs and their differentiated cells broadens the use of the hPSC paradigm in studying human cellular function and disease as well as developing therapeutics.
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