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Application of analytical standards in method development: selected chlorinated phenols and herbicides
D M Fast1, V V Reddy, D L Ashley
1Centers for Disease Control, Division of Environmental Health Laboratory Sciences, Atlanta, GA 30333.
Journal - Association of Official Analytical Chemists
|March 1, 1989
Summary
Synthesizing unavailable analytical standards, such as ethyl ethers of chlorinated phenols and phenoxy herbicides, is crucial for developing accurate analytical methods. These custom standards aid in method validation and troubleshooting, enhancing sensitivity and specificity.
Area of Science:
- Analytical Chemistry
- Organic Synthesis
Background:
- Accurate analytical methods rely on pure analytical standards.
- Commercially unavailable compounds necessitate laboratory synthesis for method development.
Purpose of the Study:
- To describe the laboratory synthesis and characterization of specific analytical standards.
- To demonstrate the utility of these standards in analytical method development and troubleshooting.
Main Methods:
- Synthesis of ethyl ethers of chlorinated phenols.
- Synthesis of ethyl esters of phenoxy herbicides.
- Characterization of synthesized compounds.
- Application of synthesized standards in tandem mass spectrometry.
Main Results:
- Successful synthesis and characterization of target compounds.
- Demonstrated use of standards for defining method characteristics.
- Utilized standards for diagnosing chromatographic and mass spectral issues.
- Obtained key mass spectral data (parent and daughter scans) for method development.
Conclusions:
- Laboratory synthesis provides essential analytical standards when unavailable commercially.
- These custom standards are vital for accurate analytical method development, validation, and problem diagnosis.
- Tandem mass spectrometry using these standards enables highly sensitive and specific analytical methods.