Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Plasmid structural instability associated with pC194 replication functions.

S Ballester1, P Lopez, M Espinosa

  • 1Centro de Investigaciones Biológicas, Consejo Superior de Investigaciones Científicas, Madrid, Spain.

Journal of Bacteriology
|May 1, 1989
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Economic evaluations of radioembolization with yttrium-90 microspheres in liver metastases of colorectal cancer: a systematic review.

BMC gastroenterology·2023
Same author

Splicing machinery is profoundly altered in systemic lupus erythematosus and antiphospholipid syndrome and directly linked to key clinical features.

Journal of autoimmunity·2023
Same author

Economic evaluations of radioembolization with Itrium-90 microspheres in hepatocellular carcinoma: a systematic review.

BMC gastroenterology·2022
Same author

A narrow intercondylar gap favours anterior cruciate ligament (ACL) rupture in patients with an immature skeleton.

Revista espanola de cirugia ortopedica y traumatologia (English ed.)·2021
Same author

Rolling away from the Wall into Granular Matter.

Physical review letters·2020
Same author

Cancer-related fatigue and its determinants in a cohort of women with breast cancer: the DAMA Cohort.

Supportive care in cancer : official journal of the Multinational Association of Supportive Care in Cancer·2020
Same journal

The bacterial SOS response promotes the expression of the transposase encoded by IS<i>CR</i> mobile genetic elements.

Journal of bacteriology·2026
Same journal

Development of a gene-editing strategy to overcome genetic intractability in <i>Lactobacillus johnsonii</i>.

Journal of bacteriology·2026
Same journal

Bactofilins are essential spatial organizers of peptidoglycan insertion in the Lyme disease spirochete <i>Borrelia burgdorferi</i>.

Journal of bacteriology·2026
Same journal

DNA damage-associated vesicle production in <i>Stenotrophomonas maltophilia</i> is mediated by the maltocin endolysin.

Journal of bacteriology·2026
Same journal

Characterization of <i>Helicobacter pylori</i> aggregation reveals a requirement for both AlpA and AlpB.

Journal of bacteriology·2026
Same journal

Cross-regulation of amino acid synthesis and anaerobic electron transfer by MetR-mediated methionine signaling.

Journal of bacteriology·2026
See all related articles

Deletions in the hybrid plasmid pJS37 enhance chloramphenicol resistance by altering gene proximity. A replication protein

Area of Science:

  • Molecular Biology
  • Genetics
  • Microbiology

Background:

  • The hybrid plasmid pJS37 combines pLS1 (streptococcal, tetracycline resistance) and pC194 (staphylococcal, chloramphenicol resistance).
  • Plasmid deletions can alter gene expression and confer novel resistance phenotypes.

Purpose of the Study:

  • To investigate the mechanism behind the generation of deleted derivatives of the hybrid plasmid pJS37.
  • To understand how these deletions influence antibiotic resistance.
  • To propose a comprehensive model for plasmid deletion formation.

Main Methods:

  • Culturing Gram-positive bacteria containing the hybrid plasmid pJS37 in the presence of chloramphenicol.
  • Analyzing the DNA sequences of accumulated deleted plasmid derivatives.

Related Experiment Videos

  • In vitro modification of the pC194 replication protein (RepH) gene.

    • Main Results:

    • Four distinct deleted derivatives of pJS37 were identified.
    • Deletions resulted in enhanced chloramphenicol resistance by repositioning the cat gene near pLS1 promoters.
    • A common deletion endpoint mapped to the RepH nicking target site, with variable endpoints showing sequence similarity.
    • Altering the RepH protein abolished this class of deletions.

    Conclusions:

    • The replication protein RepH plays a critical role in generating specific plasmid deletions.
    • Aberrant nicking activity of RepH at sequences similar to its target site can lead to deletions during rolling-circle replication.
    • A comprehensive model integrating normal and aberrant nicking events explains the formation of both previously and newly described deletion classes.