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Updated: Mar 22, 2026

Measuring Global Cellular Matrix Metalloproteinase and Metabolic Activity in 3D Hydrogels
Published on: January 22, 2019
Hyaluronic acid based hydroxamate and conjugates with biologically active amines: In vitro effect on matrix
Irina Yu Ponedel'kina1, Aigul R Gaskarova1, Elvira A Khaybrakhmanova1
1Institute of Petrochemistry and Catalysis, Russian Academy of Sciences, Ufa 450075, Russian Federation.
Abstract:
In this study, water soluble hyaluronic acid (HA) based hydroxamate and conjugates with biologically active amines and hydrazides such as p- and o-aminophenols, anthranilic, 4- and 5-aminosalicylic acids, nicotinic, N-benzylnicotinic and isonicotinic hydrazides, p-aminobenzenesulfonamide (Streptocide), p-aminobenzoic acid diethylaminoethyl ester (Procaine), and 4-amino-2,3-dimethyl-1-phenyl-3-pyrazolin-5-one (4-aminoantipyrene) were examined as matrix metalloproteinase-2 inhibitors (MMPIs). In a dose of 0.27-270μM, the most efficient MMPIs were HA conjugates with o-aminophenol=4-aminoantipyrine>4-aminosalicylic acid>5-aminosalicylic acid. Conjugates with Streptocide, Procaine and HA hydroxamate showed 40-50% inhibitory effect at all used concentrations. Conjugates with anthranilic acid and isonicotinic hydrazide (Isoniazid) in a dose of 0.27μM inhibited enzyme activity by ∼70%, but with the concentration increase their inhibitory effect was decreased.
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