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Expression of enzymatically active enkephalinase (neutral endopeptidase) in mammalian cells
C M Gorman1, D Gies, P R Schofield
1Department of Cell Genetics, Genentech, Inc., South San-Francisco, California 94080.
Journal of Cellular Biochemistry
|March 1, 1989
Abstract:
A cDNA encoding the rat enkephalinase protein (neutral endopeptidase; EC 3.4.24.11) has been constructed from overlapping lambda gt10 cDNA clones. This cDNA was inserted into an expression plasmid containing the cytomegalovirus enhancer and promoter. When transfected with this plasmid, Cos 7 cells transiently expressed the enkephalinase protein in a membrane-bound state. Recombinant enkephalinase recovered in solubilized extracts from transfected Cos 7 cells was enzymatically active and displayed properties similar to those of the native enzyme with respect to sensitivity to classical enkephalinase inhibitors.